custom ubiquitinated peptides

Our UbiQ-PEP™ service provides monoubiquitinated peptides with a range of modifications to meet your research needs. Peptides up to 20 amino acids in length are site-selectively monoubiquitinated via a native isopeptide link using our proprietary Ub ligation technology. [1,2]

 

applications:

  • epitope mapping (e.g. with deubiquitinating enzymes)
  • protein structure-function analysis
  • pull-down experiments [3]
  • ligand for X-ray analysis of proteins

 

specifications and available options with UbiQ-PEP™ Service

peptide length* up to 20 amino acids  
ubiquitinated lysine  site-selectively monoubiquitinated via a native isopeptide link

 

standard manufacturing scale**

1 mg

€3000

purity

>95% by HPLC

 

N-terminal modifications

Acetyl

Biotin

HA-tag

His6 tag

€50

€100

€500

€500

other modifications

on request

 

quality control (QC)

LC-MS and SDS-PAGE

 
standard delivery time

4 to 6 weeks

 

* please inquire about longer peptide lengths

** please inquire about bulk prices

 

 

want to discuss your project and/or receive a detailed quote?

Please contact us at sales@ubiqbio.com

 

literature

  1. El Oualid et al. "Chemical synthesis of ubiquitin, ubiquitin-based probes, and diubiquitin". Angew Chem Int Ed. 201049, 10149. http://www.ncbi.nlm.nih.gov/pubmed/21117055/
  2. F. El Oualid & H. Ovaa. "Thiolysine and selenolysine building blocks and their use in site- and chemoselective modification of peptides and proteins. 2009. U.S. Patent issued U58729009. PCT/NL2010/050277, US61/176,616, EP09160430.6
  3. Fradet-Turcotte et al. "53BP1 is a reader of the DNA-damage-induced H2A Lys 15 ubiquitin mark". Nature 2013, 499, 50. http://www.ncbi.nlm.nih.gov/pubmed/23760478