customized ubiquitinated peptides

Our UbiQ-PEP™ service provides isopeptide linked Ub-polypeptide conjugates with a range of modifications to meet your research needs. Peptides up to 20 amino acids in length are site-selectively ubiquitinated via a native isopeptide link using our proprietary Ub ligation technology. [1,2]

 

applications:

  • epitope mapping (e.g. with deubiquitinating enzymes)
  • protein structure-function analysis
  • pull-down experiments [3]
  • ligand for X-ray analysis of proteins

 

specifications and available options with UbiQ-PEP™ Service

peptide length up to 20 amino acids; * L- or D-isoforms  
ubiquitinated lysine  site-selectively monoubiquitinated via a native isopeptide link

 

manufacturing scale

100 μg

250 μg

500 μg **

€1200

€2000

€3000

purity options

crude (>75%)

HPLC purified (>95%)

75% of scale price

100% of scale price

N-terminal modifications

Acetyl

Biotin

HA-tag

His6 tag

€50

€100

€500

€350

other modifications

Possibility to incorporate special amino acids (e.g. unnatural)

on request

quality control (QC)

LC-MS

 
standard delivery time

4 to 6 weeks

 

* please inquire about longer peptide lengths

** greater amounts available upon request

*** Ahx linker between biotin and peptide

 

want to know more?

Contact us to discuss your project and/or receive a detailed quote.

+31 (0)20 303 1970

sales@ubiqbio.com 

 

literature

  1.  El Oualid et al. Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin. Angew Chem Int Ed. 49, 10149 (2010). http://www.ncbi.nlm.nih.gov/pubmed/21117055/
  2. "Thiolysine and selenolysine building blocks and their use in site-and chemoselective modification of peptides and proteins. 2009. F. El Oualid & H. Ovaa. U.S. Patent issued U58729009. PCT/NL2010/050277, US61/176,616, EP09160430.6
  3. Fradet-Turcotte et al. 53BP1 is a reader of the DNA-damage-induced H2A Lys 15 ubiquitin mark. Nature 499, 50-54 (2013). http://www.ncbi.nlm.nih.gov/pubmed/23760478