want to know more?
+31 (0)20 303 1970

Conserved from yeast to human, the histone methyltransferase Dot1 methylates lysine 79 of histone H3 (H3K79) on the nucleosome core. This methylation affects gene expression and DNA damage response, and is enhanced by monoubiquitination of the C-terminus of histone H2B [1 - 4]. Given the promising clinical development of Dot1 inhibitors (e.g. for the treatment of acute leukemia in children [5]), it is important to elucidate the regulatory mechanisms of Dot1 [1 - 4].......(more)

 

Read More

A recent paper in Nature Communications describes a collaborative effort between scientists at UbiQ, Genentech, Boston Biochem (Bio-Techne) and Stanford University (1). Here a reactive-site-centric chemoproteomics protocol is presented which allows evaluating activity and probe reactivity of deubiquitinating enzymes (DUBs, also termed deubiquitylases)......(more)

 

Read More

Hereby we present UbiQ-121 as an activity-based probe for OTULIN, a deubiquitinating enzyme that is specific for Met1 linked ubiquitin chains and acts as a negative regulator of nuclear factor κB signaling and immune homeostasis. UbiQ-121 is based on Met1 linked diubiquitin in which G76 of the distal ubiquitin is replaced by an electrophilic dehydroalanine (Dha) residue. The catalytic cysteine residue of OTULIN reacts in a covalent manner with the Dha residue and as such UbiQ-121 captures OTULIN in its active conformation .....(more)

 

Read More

Ub photoLeu73 (UbiQ-154), a new and powerful crosslinkable Ub. UbiQ-154 is a new crosslinking reagent based on ubiquitin in which Leu73 is replaced by the photoreactive analog photoleucine.....(more)

 

Read More