reagents

Biotin-Ahx-SUMO2-Dha (Dha= dehydroalanine) is a new and first of its kind activity based probe for the SUMO ligation pathway. [

UbiQ-023 is a fluorescence polarization HTS control reagent that can be used to make a concentration calibration curve and determine the grating factor (G).

His10-SUMO3-Dha is a new and first of its kind activity based probe for the SUMO ligation pathway.

UbiQ-158 is an activity-based probe for E1 activating enzymes and E2 conjugating enzymes that are involved in autophagy (e.g. ATG7 and ATG3, respectively). The N-terminus is labeled with an His6 affinity tag and a 3C protease cleavage site (QG), Cys17 has been mutated to a Ser residue (S).

Biotin-Ahx-SUMO2-VME is an activity-based probe for SUMO proteases.

Biotin-ANP-Ub-PA (UbiQ-077) is a newly developed potent and specific inhibitor of deubiquitylating enzymes (DUBs). It is labeled on the N-terminus with biotin and contains the UV cleavable 3-amino-3-(2-nitrophenyl)propanoic acid (ANP) linker, allowing for UV mediated cleavage of the biotin tag.

human SUMO2 sequence (C48S) which is functionalized on the N-terminus with a 5-carboxyrhodamine110 dye.

UbiQ-174 consists of the ubiquitin protein (Ub) in which Lys6 has been mutated to a cysteine residue.

UbiQ-175 consists of the ubiquitin protein (Ub) in which Lys11 has been mutated to a cysteine residue.

UbiQ-041 (K382 Ub-p53(368-389)-FP) is a class II fluorescence polarization HTS reagent based on the C-terminal peptide sequence 368 – 389 of p53, which contains various lysine residues that are (mono)ubiquitinated.

UbiQ-041 (K382 Ub-p53(368-389)-FP) is a class II fluorescence polarization HTS reagent based on the C-terminal peptide sequence 368 – 389 of p53, which contains various lysine residues that are (mono)ubiquitinated.

Potent, irreversible and specific inhibitor based on K63 linked diUb that targets the S2 pocket of deubiquitinating enzymes (DUBs). The C-terminus of the proximal Ub is equiped with the propargylamide (PA) electrophile and the isopeptide bond between the two Ub proteins is replaced by a DUB resistant mimic.

SUMO1-Dha is a new and first of its kind activity based probe for the SUMO ligation pathway.

SUMO2-Dha is a new and first of its kind activity based probe for the SUMO ligation pathway.

SUMO3-Dha is a new and first of its kind activity based probe for the SUMO ligation pathway.