reagents
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Biotin-Ahx-SUMO2-Dha
UbiQ code: UbiQ-159Biotin-Ahx-SUMO2-Dha (Dha= dehydroalanine) is a new and first of its kind activity based probe for the SUMO ligation pathway. [
crystallizationpull downpurificationwestern blotphenotypic protein profilingtarget inhibitor profiling -
5-TAMRA-Lys-Gly-OH
UbiQ code: UbiQ-023UbiQ-023 is a fluorescence polarization HTS control reagent that can be used to make a concentration calibration curve and determine the grating factor (G).
compound library screening -
His10-SUMO3-Dha
UbiQ code: UbiQ-124His10-SUMO3-Dha is a new and first of its kind activity based probe for the SUMO ligation pathway.
crystallizationwestern blot -
His6-3C-MAP1LC3a-Dha
UbiQ code: UbiQ-158UbiQ-158 is an activity-based probe for E1 activating enzymes and E2 conjugating enzymes that are involved in autophagy (e.g. ATG7 and ATG3, respectively). The N-terminus is labeled with an His6 affinity tag and a 3C protease cleavage site (QG), Cys17 has been mutated to a Ser residue (S).
crystallizationwestern blot -
Biotin-Ahx-SUMO2-VME
UbiQ code: UbiQ-156Biotin-Ahx-SUMO2-VME is an activity-based probe for SUMO proteases.
crystallizationpull downpurificationwestern blotphenotypic protein profilingDUB activity profilingtarget inhibitor profiling -
Biotin-ANP-Ub-PA
UbiQ code: UbiQ-077Biotin-ANP-Ub-PA (UbiQ-077) is a newly developed potent and specific inhibitor of deubiquitylating enzymes (DUBs). It is labeled on the N-terminus with biotin and contains the UV cleavable 3-amino-3-(2-nitrophenyl)propanoic acid (ANP) linker, allowing for UV mediated cleavage of the biotin tag.
crystallizationpull downpurificationwestern blotphenotypic protein profilingDUB activity profilingtarget inhibitor profiling -
5-carboxyRh110-SUMO2
UbiQ code: UbiQ-134human SUMO2 sequence (C48S) which is functionalized on the N-terminus with a 5-carboxyrhodamine110 dye.
crystallizationMSphenotypic protein profilingtarget inhibitor profiling -
Ub K6C
UbiQ code: UbiQ-174UbiQ-174 consists of the ubiquitin protein (Ub) in which Lys6 has been mutated to a cysteine residue.
DUB activity profilingcompound library screeningtarget inhibitor profiling -
Ub K11C
UbiQ code: UbiQ-175UbiQ-175 consists of the ubiquitin protein (Ub) in which Lys11 has been mutated to a cysteine residue.
DUB activity profilingcompound library screeningtarget inhibitor profiling -
K382 Ub-p53(357-389)-FP
UbiQ code: UbiQ-042UbiQ-041 (K382 Ub-p53(368-389)-FP) is a class II fluorescence polarization HTS reagent based on the C-terminal peptide sequence 368 – 389 of p53, which contains various lysine residues that are (mono)ubiquitinated.
DUB activity profilingcompound library screening -
K382 Ub-p53(368-389)-FP
UbiQ code: UbiQ-041UbiQ-041 (K382 Ub-p53(368-389)-FP) is a class II fluorescence polarization HTS reagent based on the C-terminal peptide sequence 368 – 389 of p53, which contains various lysine residues that are (mono)ubiquitinated.
DUB activity profilingcompound library screening -
K63 diUb PA
UbiQ code: UbiQ-114Potent, irreversible and specific inhibitor based on K63 linked diUb that targets the S2 pocket of deubiquitinating enzymes (DUBs). The C-terminus of the proximal Ub is equiped with the propargylamide (PA) electrophile and the isopeptide bond between the two Ub proteins is replaced by a DUB resistant mimic.
crystallizationpull downpurificationwestern blotphenotypic protein profiling -
SUMO1-Dha
UbiQ code: UbiQ-116SUMO1-Dha is a new and first of its kind activity based probe for the SUMO ligation pathway.
crystallizationwestern blot -
SUMO2-Dha
UbiQ code: UbiQ-117SUMO2-Dha is a new and first of its kind activity based probe for the SUMO ligation pathway.
crystallizationwestern blot -
SUMO3-Dha
UbiQ code: UbiQ-118SUMO3-Dha is a new and first of its kind activity based probe for the SUMO ligation pathway.
crystallizationwestern blot