reagents
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SUMO2-Dha
UbiQ code: UbiQ-117SUMO2-Dha is a new and first of its kind activity based probe for the SUMO ligation pathway.
crystallizationwestern blot -
His10-SUMO3-Dha
UbiQ code: UbiQ-124His10-SUMO3-Dha is a new and first of its kind activity based probe for the SUMO ligation pathway.
crystallizationwestern blot -
Ub-PA
UbiQ code: UbiQ-057Ubiquitin-propargylamide is a newly developed potent and specific inhibitor of deubiquitylating enzymes (DUBs).
crystallizationpull downpurificationwestern blotMSNMRphenotypic protein profiling -
K63 diUb PA
UbiQ code: UbiQ-114Potent, irreversible and specific inhibitor based on K63 linked diUb that targets the S2 pocket of deubiquitinating enzymes (DUBs). The C-terminus of the proximal Ub is equiped with the propargylamide (PA) electrophile and the isopeptide bond between the two Ub proteins is replaced by a DUB resistant mimic.
crystallizationpull downpurificationwestern blotphenotypic protein profiling -
HA-Ahx-Ahx-Ub-VS
UbiQ code: UbiQ-187HA-Ahx-Ahx-Ub-VS (UbiQ-187) is a potent and specific inhibitor of deubiquitinating enzymes (DUBs) based on a C-terminal electrophilic vinyl sulfone (VS) group.
crystallizationpurificationwestern blotphenotypic protein profilingDUB activity profiling -
Biotin-ANP-Ub-PA
UbiQ code: UbiQ-077Biotin-ANP-Ub-PA (UbiQ-077) is a newly developed potent and specific inhibitor of deubiquitylating enzymes (DUBs). It is labeled on the N-terminus with biotin and contains the UV cleavable 3-amino-3-(2-nitrophenyl)propanoic acid (ANP) linker, allowing for UV mediated cleavage of the biotin tag.
crystallizationpull downpurificationwestern blotphenotypic protein profilingDUB activity profilingtarget inhibitor profiling -
His6-Ahx-Ahx-SUMO2-VPS
UbiQ code: UbiQ-237His6-Ahx-Ahx-SUMO2-VPS (UbiQ-237) is an activity-based probe for SUMO proteases.
crystallizationpull downpurificationwestern blotMSNMRphenotypic protein profilingDUB activity profilingcompound library screeningtarget inhibitor profiling -
SUMO3-Dha
UbiQ code: UbiQ-118SUMO3-Dha is a new and first of its kind activity based probe for the SUMO ligation pathway.
crystallizationwestern blot -
His6-3C-MAP1LC3a-Dha
UbiQ code: UbiQ-158UbiQ-158 is an activity-based probe for E1 activating enzymes and E2 conjugating enzymes that are involved in autophagy (e.g. ATG7 and ATG3, respectively). The N-terminus is labeled with an His6 affinity tag and a 3C protease cleavage site (QG), Cys17 has been mutated to a Ser residue (S).
crystallizationwestern blot -
Ub-VME
UbiQ code: UbiQ-005Ub-VME is a potent, irreversible and specific inhibitor of deubiquitylating enzymes (DUBs),
crystallizationpull downpurificationwestern blotMSNMRphenotypic protein profilingDUB activity profilingcompound library screeningtarget inhibitor profiling -
SUMO1-Dha
UbiQ code: UbiQ-116SUMO1-Dha is a new and first of its kind activity based probe for the SUMO ligation pathway.
crystallizationwestern blot -
Biotin-K63 diUb-VME
UbiQ code: UbiQ-115UbiQ-115 (Biotin-Ahx-K63 diUbiquitin VME) probe is a potent, irreversible and specific inhibitor of deubiquitylating enzymes (DUBs) based on K63 linked diUb. Here Lys63 has been replaced by a diaminobutyric acid residue equipped with a VME type warhead3.
crystallizationpull downpurificationwestern blotphenotypic protein profiling -
Ub photoLeu73
UbiQ code: UbiQ-154UbiQ-154 is a new crosslinking reagent based on ubiquitin in which Leu73 is replaced by the photoreactive analog photoleucine (photoLeu).
crystallizationpull downwestern blotMS -
Ac-ISG15prox-VPS
UbiQ code: UbiQ-262UbiQ-262 is an activity-based probe for and inhibitor of ISG15 proteases.
crystallizationpull downpurificationwestern blotMSNMRphenotypic protein profilingDUB activity profilingcompound library screeningtarget inhibitor profiling -
Biotin-Ahx-SUMO2-Dha
UbiQ code: UbiQ-159Biotin-Ahx-SUMO2-Dha (Dha= dehydroalanine) is a new and first of its kind activity based probe for the SUMO ligation pathway. [
crystallizationpull downpurificationwestern blotphenotypic protein profilingtarget inhibitor profiling