reagents

Biotin-Ahx-SUMO2-VME is an activity-based probe for SUMO proteases.

HA-Ahx-Ahx-Ub-VPS (UbiQ-193) is an activity-based probe and inhibitor of DUBs.

SUMO2-Dha is a new and first of its kind activity based probe for the SUMO ligation pathway.

His10-SUMO3-Dha is a new and first of its kind activity based probe for the SUMO ligation pathway.

UbiQ-057 (Ub-PA) is an activity-based probe for deubiquitinating enzymes (DUBs) and contains propargyl amide (PA) as an electrophile.

Potent, irreversible and specific inhibitor based on K63 linked diUb that targets the S2 pocket of deubiquitinating enzymes (DUBs). The C-terminus of the proximal Ub is equipped with the propargyl amide (PA) electrophile and the isopeptide bond between the two Ub proteins is replaced by a DUB resistant mimic.

HA-Ahx-Ahx-Ub-VS (UbiQ-187) is a potent and specific inhibitor of deubiquitinating enzymes (DUBs) based on a C-terminal electrophilic vinyl sulfone (VS) group.

Biotin-ANP-Ub-PA (UbiQ-077) is a newly developed potent and specific inhibitor of deubiquitylating enzymes (DUBs). It is labeled on the N-terminus with biotin and contains the UV cleavable 3-amino-3-(2-nitrophenyl)propanoic acid (ANP) linker, allowing for UV mediated cleavage of the biotin tag.

His6-Ahx-Ahx-SUMO2-VPS (UbiQ-237) is an activity-based probe for SUMO proteases.

SUMO3-Dha is a new and first of its kind activity based probe for the SUMO ligation pathway.

UbiQ-158 is an activity-based probe for E1 activating enzymes and E2 conjugating enzymes that are involved in autophagy (e.g. ATG7 and ATG3, respectively). The N-terminus is labeled with an His6 affinity tag and a 3C protease cleavage site (QG), Cys17 has been mutated to a Ser residue (S).

Ub-VME is a potent, irreversible and specific inhibitor of deubiquitylating enzymes (DUBs),

SUMO1-Dha is a new and first of its kind activity based probe for the SUMO ligation pathway.

UbiQ-115 (Biotin-Ahx-K63 diUbiquitin VME) probe is a potent, irreversible and specific inhibitor of deubiquitylating enzymes (DUBs) based on K63 linked diUb. Here Lys63 has been replaced by a diaminobutyric acid residue equipped with a VME type warhead3.

UbiQ-154 is a new crosslinking reagent based on ubiquitin in which Leu73 is replaced by the photoreactive analog photoleucine (photoLeu).