reagents
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Biotin-Ahx-Ub pTyr59
UbiQ code: UbiQ-095UbiQ-095 is ubiquitin that is phosphorylated on Tyr59.
pull downpurificationphenotypic protein profilingcompound library screening -
Biotin-K63 diUb-VME
UbiQ code: UbiQ-115UbiQ-115 (Biotin-Ahx-K63 diUbiquitin VME) probe is a potent, irreversible and specific inhibitor of deubiquitylating enzymes (DUBs) based on K63 linked diUb. Here Lys63 has been replaced by a diaminobutyric acid residue equipped with a VME type warhead3.
crystallizationpull downpurificationwestern blotphenotypic protein profiling -
Biotin-Ahx-Ub pThr12
UbiQ code: UbiQ-093UbiQ-093 is ubiquitin that is phosphorylated on Thr12.
pull downpurificationphenotypic protein profilingcompound library screening -
Ub-PA
UbiQ code: UbiQ-057Ubiquitin-propargylamide is a newly developed potent and specific inhibitor of deubiquitylating enzymes (DUBs).
crystallizationpull downpurificationwestern blotMSNMRphenotypic protein profiling -
Biotin-Ahx-SUMO2-Dha
UbiQ code: UbiQ-159Biotin-Ahx-SUMO2-Dha (Dha= dehydroalanine) is a new and first of its kind activity based probe for the SUMO ligation pathway. [
crystallizationpull downpurificationwestern blotphenotypic protein profilingtarget inhibitor profiling -
Biotin-Ahx-Ub pThr7
UbiQ code: UbiQ-092UbiQ-092 is a ubiquitin protein that is phosphorylated on Thr7. Phosphoproteomic studies have identified several phosphorylated sites in ubiquitin, among them pThr7.
pull downpurificationphenotypic protein profilingcompound library screening -
Ub pSer57
UbiQ code: UbiQ-113UbiQ-113 is ubiquitin that is phosphorylated on Ser57.
pull downpurificationphenotypic protein profilingcompound library screening -
Biotin-Ahx-SUMO2-VME
UbiQ code: UbiQ-156Biotin-Ahx-SUMO2-VME is an activity-based probe for SUMO proteases.
crystallizationpull downpurificationwestern blotphenotypic protein profilingDUB activity profilingtarget inhibitor profiling -
Biotin-Ahx-Ub pSer 57
UbiQ code: UbiQ-094UbiQ-094 is ubiquitin that is phosphorylated on Ser57.
pull downpurificationphenotypic protein profilingcompound library screening -
Ub-VME
UbiQ code: UbiQ-005Ub-VME is a potent, irreversible and specific inhibitor of deubiquitylating enzymes (DUBs),
crystallizationpull downpurificationwestern blotMSNMRphenotypic protein profilingDUB activity profilingcompound library screeningtarget inhibitor profiling -
K63 diUb PA
UbiQ code: UbiQ-114Potent, irreversible and specific inhibitor based on K63 linked diUb that targets the S2 pocket of deubiquitinating enzymes (DUBs). The C-terminus of the proximal Ub is equiped with the propargylamide (PA) electrophile and the isopeptide bond between the two Ub proteins is replaced by a DUB resistant mimic.
crystallizationpull downpurificationwestern blotphenotypic protein profiling -
Biotin-Ahx-Ub pSer 65
UbiQ code: UbiQ-091UbiQ-091 is ubiquitin that is phosphorylated on Ser65, a ubiquitin variant which has been shown to activate Parkin E3 ligase mediated ubiquitination.
pull downpurificationphenotypic protein profilingcompound library screening -
Ub photoLeu73
UbiQ code: UbiQ-154UbiQ-154 is a new crosslinking reagent based on ubiquitin in which Leu73 is replaced by the photoreactive analog photoleucine (photoLeu).
crystallizationpull downwestern blotMS -
Biotin-ANP-Ub-PA
UbiQ code: UbiQ-077Biotin-ANP-Ub-PA (UbiQ-077) is a newly developed potent and specific inhibitor of deubiquitylating enzymes (DUBs). It is labeled on the N-terminus with biotin and contains the UV cleavable 3-amino-3-(2-nitrophenyl)propanoic acid (ANP) linker, allowing for UV mediated cleavage of the biotin tag.
crystallizationpull downpurificationwestern blotphenotypic protein profilingDUB activity profilingtarget inhibitor profiling -
DUB activity based probe explorer panel
UbiQ code: UbiQ-L02UbiQ-L02 is a panel of the following 10 deubiquitylating enzyme (DUB) activity based probes (ABPs).
crystallizationpull downpurificationwestern blotMSNMRphenotypic protein profiling
