reagents

Biotin-ANP-Ub-PA (UbiQ-077) is a newly developed potent and specific inhibitor of deubiquitylating enzymes (DUBs). It is labeled on the N-terminus with biotin and contains the UV cleavable 3-amino-3-(2-nitrophenyl)propanoic acid (ANP) linker, allowing for UV mediated cleavage of the biotin tag.

HA-Ahx-Ahx-Ub-VS (UbiQ-187) is a potent and specific inhibitor of deubiquitinating enzymes (DUBs) based on a C-terminal electrophilic vinyl sulfone (VS) group.

Ub-VME is a potent, irreversible and specific inhibitor of deubiquitylating enzymes (DUBs),

Potent, irreversible and specific inhibitor based on K63 linked diUb that targets the S2 pocket of deubiquitinating enzymes (DUBs). The C-terminus of the proximal Ub is equiped with the propargylamide (PA) electrophile and the isopeptide bond between the two Ub proteins is replaced by a DUB resistant mimic.

UbiQ-154 is a new crosslinking reagent based on ubiquitin in which Leu73 is replaced by the photoreactive analog photoleucine (photoLeu).

UbiQ-115 (Biotin-Ahx-K63 diUbiquitin VME) probe is a potent, irreversible and specific inhibitor of deubiquitylating enzymes (DUBs) based on K63 linked diUb. Here Lys63 has been replaced by a diaminobutyric acid residue equipped with a VME type warhead3.

Biotin-Ahx-Ub-VS (UbiQ-188) is a potent and specific inhibitor of deubiquitinating enzymes (DUBs) based on a C-terminal electrophilic vinyl sulfone (VS) group.

Ubiquitin-propargylamide is a newly developed potent and specific inhibitor of deubiquitylating enzymes (DUBs).

UbiQ-L01 is a panel of all eight native linked di-ubiquitin (diUb) conjugates: M1 (i.e. linear), K6, K11, K27, K29, K33, K48 and K63 linked.

UbiQ-L02 is a panel of 10 deubiquitinating enzyme (DUB) activity-based probes (ABPs, 10 micrograms each).

Ubiquitin(-like) (e.g. Ub, Nedd8, SUMO, etc.) assay reagents with various modifications (unnatural amino acids, peptides, tags, etc.)

UbiQ-L04 contains seven deubiquitylating enzymes (DUB) activity-based probes based on K6, 11, 27, 29, 33, 48, and 63 linked diUb.

Ubiquitin-Viny Sulfone is an inhibitor of deubiquitylating enzymes (DUBs).

This probe is a potent and specific inhibitor of deubiquitylating enzymes (DUBs) containing the propargylamide (PA) warhead.

UbiQ-035 (Ub-VME) is a potent, irreversible and specific inhibitor of deubiquitylating enzymes (DUBs), which is N-terminally tagged with an HA-tag.