reagents
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DUB assay explorer panel
UbiQ code: UbiQ-L03UbiQ-L03 is a panel of 4 deubiquitylating enzyme (DUB) activity assay reagents (25 ug each) prepared by total chemical synthesis.
DUB activity profilingcompound library screening -
custom assay reagents
UbiQ code: UbiQ-C01 ref POUbiquitin(-like) (e.g. Ub, Nedd8, SUMO, etc.) assay reagents with various modifications (unnatural amino acids, peptides, tags, etc.)
crystallizationpull downpurificationwestern blotMSNMRphenotypic protein profilingDUB activity profilingcompound library screeningtarget inhibitor profiling -
Ub K48C
UbiQ code: UbiQ-179UbiQ-179 consists of the ubiquitin protein (Ub) in which Lys48 has been mutated to a cysteine residue.
DUB activity profilingcompound library screeningtarget inhibitor profiling -
K382 Ub-p53(368-389)-FP
UbiQ code: UbiQ-041UbiQ-041 (K382 Ub-p53(368-389)-FP) is a class II fluorescence polarization HTS reagent based on the C-terminal peptide sequence 368 – 389 of p53, which contains various lysine residues that are (mono)ubiquitinated.
DUB activity profilingcompound library screening -
K382 Ub-p53(357-389)-FP
UbiQ code: UbiQ-042UbiQ-041 (K382 Ub-p53(368-389)-FP) is a class II fluorescence polarization HTS reagent based on the C-terminal peptide sequence 368 – 389 of p53, which contains various lysine residues that are (mono)ubiquitinated.
DUB activity profilingcompound library screening -
Ub K6C
UbiQ code: UbiQ-174UbiQ-174 consists of the ubiquitin protein (Ub) in which Lys6 has been mutated to a cysteine residue.
DUB activity profilingcompound library screeningtarget inhibitor profiling -
Ub K11C
UbiQ code: UbiQ-175UbiQ-175 consists of the ubiquitin protein (Ub) in which Lys11 has been mutated to a cysteine residue.
DUB activity profilingcompound library screeningtarget inhibitor profiling -
Ub K27C
UbiQ code: UbiQ-176UbiQ-176 consists of the ubiquitin protein (Ub) in which Lys27 has been mutated to a cysteine residue.
DUB activity profilingcompound library screeningtarget inhibitor profiling -
Ub K29C
UbiQ code: UbiQ-177UbiQ-177 consists of the ubiquitin protein (Ub) in which Lys29 has been mutated to a cysteine residue.
DUB activity profilingcompound library screeningtarget inhibitor profiling -
Ub K33C
UbiQ code: UbiQ-178UbiQ-178 consists of the ubiquitin protein (Ub) in which Lys33 has been mutated to a cysteine residue.
DUB activity profilingcompound library screeningtarget inhibitor profiling -
Ub K63C
UbiQ code: UbiQ-180UbiQ-180 consists of the ubiquitin protein (Ub) in which Lys63 has been mutated to a cysteine residue.
DUB activity profilingcompound library screeningtarget inhibitor profiling -
hISG15-FP
UbiQ code: UbiQ-287UbiQ-287 is a fluorescence polarization assay reagent for deISGylating enzymes which is based on a 5-carboxytetramethylrhodamine (TAMRA, exc 550 nm, emi 590 nm) modified Lys-Gly sequence that is linked to human ISG15 via a native isopeptide bond.
DUB activity profilingcompound library screening -
Ub-aminoluciferin
UbiQ code: UbiQ-036Ub-aminoluciferin (UbiQ-036) is a DUB activity assay reagent based on ubiquitin that is C-terminally functionalized with 6-aminoluciferin.
DUB activity profilingcompound library screening -
Ub-AMC
UbiQ code: UbiQ-001UbiQ-001 (Ub-AMC) is a quenched, fluorescent substrate for ubiquitin proteases.
DUB activity profilingcompound library screening -
Ub-Rh110Gly
UbiQ code: UbiQ-002Ubiquitin-Rhodamine110Gly (Ub-Rh110Gly) (UbiQ-002) is a quenched, fluorescent substrate for ubiquitin proteases.
DUB activity profilingcompound library screening