reagents

  • Ub K29C

    UbiQ code: UbiQ-177

    UbiQ-177 consists of the ubiquitin protein (Ub) in which Lys29 has been mutated to a cysteine residue.

    DUB activity profilingcompound library screeningtarget inhibitor profiling
  • Ub K48C

    UbiQ code: UbiQ-179

    UbiQ-179 consists of the ubiquitin protein (Ub) in which Lys48 has been mutated to a cysteine residue.

    DUB activity profilingcompound library screeningtarget inhibitor profiling
  • Ub K11C

    UbiQ code: UbiQ-175

    UbiQ-175 consists of the ubiquitin protein (Ub) in which Lys11 has been mutated to a cysteine residue.

    DUB activity profilingcompound library screeningtarget inhibitor profiling
  • 5-carboxyRh110-SUMO2

    UbiQ code: UbiQ-134

    human SUMO2 sequence (C48S) which is functionalized on the N-terminus with a 5-carboxyrhodamine110 dye.

    crystallizationMSphenotypic protein profilingtarget inhibitor profiling
  • Ub K33C

    UbiQ code: UbiQ-178

    UbiQ-178 consists of the ubiquitin protein (Ub) in which Lys33 has been mutated to a cysteine residue.

    DUB activity profilingcompound library screeningtarget inhibitor profiling
  • Biotin-Ahx-SUMO2-VME

    UbiQ code: UbiQ-156

    Biotin-Ahx-SUMO2-VME is an activity-based probe for SUMO proteases.

    crystallizationpull downpurificationwestern blotphenotypic protein profilingDUB activity profilingtarget inhibitor profiling
  • Ub K27C

    UbiQ code: UbiQ-176

    UbiQ-176 consists of the ubiquitin protein (Ub) in which Lys27 has been mutated to a cysteine residue.

    DUB activity profilingcompound library screeningtarget inhibitor profiling
  • Biotin-Ahx-SUMO2-Dha

    UbiQ code: UbiQ-159

    Biotin-Ahx-SUMO2-Dha (Dha= dehydroalanine) is a new and first of its kind activity based probe for the SUMO ligation pathway. [

    crystallizationpull downpurificationwestern blotphenotypic protein profilingtarget inhibitor profiling
  • Ub K6C

    UbiQ code: UbiQ-174

    UbiQ-174 consists of the ubiquitin protein (Ub) in which Lys6 has been mutated to a cysteine residue.

    DUB activity profilingcompound library screeningtarget inhibitor profiling
  • Ub K63C

    UbiQ code: UbiQ-180

    UbiQ-180 consists of the ubiquitin protein (Ub) in which Lys63 has been mutated to a cysteine residue.

    DUB activity profilingcompound library screeningtarget inhibitor profiling
  • Biotin-ANP-Ub-PA

    UbiQ code: UbiQ-077

    Biotin-ANP-Ub-PA (UbiQ-077) is a newly developed potent and specific inhibitor of deubiquitylating enzymes (DUBs). It is labeled on the N-terminus with biotin and contains the UV cleavable 3-amino-3-(2-nitrophenyl)propanoic acid (ANP) linker, allowing for UV mediated cleavage of the biotin tag.

    crystallizationpull downpurificationwestern blotphenotypic protein profilingDUB activity profilingtarget inhibitor profiling
  • Ub-FP

    UbiQ code: UbiQ-012

    UbiQ-012 (Ub-FP) is a fluorescence polarization assay reagent based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to ubiquitin via a native isopeptide bond with the lysine side-chain

    DUB activity profilingcompound library screeningtarget inhibitor profiling
  • His6-Ahx-Ahx-Ub-Dha

    UbiQ code: UbiQ-103

    UbiQ-103 is a new activity based probe for Ub E1, E2 and (HECT/RBR) E3 ligases. The N-terminus of UbiQ-103 is labeled with an His6 tag; two aminohexanoic acid (Ahx) linkers are used to create extra space between the His6 and Ub protein for efficient access of His6 binding entities.

    crystallizationpull downpurificationwestern blotphenotypic protein profilingtarget inhibitor profiling
  • Biotin-Ahx-Ub-Dha

    UbiQ code: UbiQ-102

    Biotin-Ahx-Ub-Dha is a new activity based probe for Ub E1, E2 and (HECT/RBR) E3 ligases including a biotin tag.

    crystallizationpull downpurificationwestern blotphenotypic protein profilingtarget inhibitor profiling
  • 5-carboxyRh110-Ub-Dha

    UbiQ code: UbiQ-131

    5-carboxyRh110-Ub-Dha is a new activity based probe for Ub E1, E2 and (HECT/RBR) E3 ligases.

    phenotypic protein profilingcompound library screeningtarget inhibitor profiling

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