reagents
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5-TAMRA-Lys-Gly-OH
UbiQ code: UbiQ-023UbiQ-023 is a fluorescence polarization HTS control reagent that can be used to make a concentration calibration curve and determine the grating factor (G).
compound library screening -
Ub K33C
UbiQ code: UbiQ-178UbiQ-178 consists of the ubiquitin protein (Ub) in which Lys33 has been mutated to a cysteine residue.
DUB activity profilingcompound library screeningtarget inhibitor profiling -
Biotin-Ahx-Ub pSer 57
UbiQ code: UbiQ-094UbiQ-094 is ubiquitin that is phosphorylated on Ser57.
pull downpurificationphenotypic protein profilingcompound library screening -
His6-Ahx-Ahx-SUMO2-VPS
UbiQ code: UbiQ-237His6-Ahx-Ahx-SUMO2-VPS (UbiQ-237) is an activity-based probe for SUMO proteases.
crystallizationpull downpurificationwestern blotMSNMRphenotypic protein profilingDUB activity profilingcompound library screeningtarget inhibitor profiling -
K382 Ub-p53(368-389)-FP
UbiQ code: UbiQ-041UbiQ-041 (K382 Ub-p53(368-389)-FP) is a class II fluorescence polarization HTS reagent based on the C-terminal peptide sequence 368 – 389 of p53, which contains various lysine residues that are (mono)ubiquitinated.
DUB activity profilingcompound library screening -
Ub K27C
UbiQ code: UbiQ-176UbiQ-176 consists of the ubiquitin protein (Ub) in which Lys27 has been mutated to a cysteine residue.
DUB activity profilingcompound library screeningtarget inhibitor profiling -
Biotin-Ahx-Ub pSer 65
UbiQ code: UbiQ-091UbiQ-091 is ubiquitin that is phosphorylated on Ser65, a ubiquitin variant which has been shown to activate Parkin E3 ligase mediated ubiquitination.
pull downpurificationphenotypic protein profilingcompound library screening -
Ub-VME
UbiQ code: UbiQ-005Ub-VME is a potent, irreversible and specific inhibitor of deubiquitylating enzymes (DUBs),
crystallizationpull downpurificationwestern blotMSNMRphenotypic protein profilingDUB activity profilingcompound library screeningtarget inhibitor profiling -
Ub K6C
UbiQ code: UbiQ-174UbiQ-174 consists of the ubiquitin protein (Ub) in which Lys6 has been mutated to a cysteine residue.
DUB activity profilingcompound library screeningtarget inhibitor profiling -
Ub K63C
UbiQ code: UbiQ-180UbiQ-180 consists of the ubiquitin protein (Ub) in which Lys63 has been mutated to a cysteine residue.
DUB activity profilingcompound library screeningtarget inhibitor profiling -
Biotin-Ahx-Ub pTyr59
UbiQ code: UbiQ-095UbiQ-095 is ubiquitin that is phosphorylated on Tyr59.
pull downpurificationphenotypic protein profilingcompound library screening -
K382 Ub-p53(357-389)-FP
UbiQ code: UbiQ-042UbiQ-041 (K382 Ub-p53(368-389)-FP) is a class II fluorescence polarization HTS reagent based on the C-terminal peptide sequence 368 – 389 of p53, which contains various lysine residues that are (mono)ubiquitinated.
DUB activity profilingcompound library screening -
Ub K29C
UbiQ code: UbiQ-177UbiQ-177 consists of the ubiquitin protein (Ub) in which Lys29 has been mutated to a cysteine residue.
DUB activity profilingcompound library screeningtarget inhibitor profiling -
Biotin-Ahx-Ub pThr12
UbiQ code: UbiQ-093UbiQ-093 is ubiquitin that is phosphorylated on Thr12.
pull downpurificationphenotypic protein profilingcompound library screening -
HA-Ahx-Ahx-Ub-VPS
UbiQ code: UbiQ-193HA-Ahx-Ahx-Ub-VPS (UbiQ-193) is an activity-based probe and inhibitor of DUBs.
crystallizationpull downpurificationwestern blotMSNMRphenotypic protein profilingDUB activity profilingcompound library screeningtarget inhibitor profiling
