reagents
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DUB activity-based probe explorer panel
UbiQ code: UbiQ-L02UbiQ-L02 is a panel of 10 deubiquitinating enzyme (DUB) activity-based probes (ABPs, 10 micrograms each).
crystallizationpull downpurificationwestern blotMSNMRphenotypic protein profiling -
di-ubiquitin activity based probe explorer panel
UbiQ code: UbiQ-L04UbiQ-L04 contains seven deubiquitylating enzymes (DUB) activity-based probes based on K6, 11, 27, 29, 33, 48, and 63 linked diUb.
crystallizationpurification -
His6-3C-MAP1LC3a-Dha
UbiQ code: UbiQ-158UbiQ-158 is an activity-based probe for E1 activating enzymes and E2 conjugating enzymes that are involved in autophagy (e.g. ATG7 and ATG3, respectively). The N-terminus is labeled with an His6 affinity tag and a 3C protease cleavage site (QG), Cys17 has been mutated to a Ser residue (S).
crystallizationwestern blot -
Biotin-Ahx-SUMO2-VME
UbiQ code: UbiQ-156Biotin-Ahx-SUMO2-VME is an activity-based probe for SUMO proteases.
crystallizationpull downpurificationwestern blotphenotypic protein profilingDUB activity profilingtarget inhibitor profiling -
Biotin-ANP-Ub-PA
UbiQ code: UbiQ-077Biotin-ANP-Ub-PA (UbiQ-077) is a newly developed potent and specific inhibitor of deubiquitylating enzymes (DUBs). It is labeled on the N-terminus with biotin and contains the UV cleavable 3-amino-3-(2-nitrophenyl)propanoic acid (ANP) linker, allowing for UV mediated cleavage of the biotin tag.
crystallizationpull downpurificationwestern blotphenotypic protein profilingDUB activity profilingtarget inhibitor profiling -
Ub-PA
UbiQ code: UbiQ-057Ubiquitin-propargylamide is a newly developed potent and specific inhibitor of deubiquitylating enzymes (DUBs).
crystallizationpull downpurificationwestern blotMSNMRphenotypic protein profiling -
Ub-VME
UbiQ code: UbiQ-005Ub-VME is a potent, irreversible and specific inhibitor of deubiquitylating enzymes (DUBs),
crystallizationpull downpurificationwestern blotMSNMRphenotypic protein profilingDUB activity profilingcompound library screeningtarget inhibitor profiling -
HA-Ahx-Ahx-Ub-VPS
UbiQ code: UbiQ-193HA-Ahx-Ahx-Ub-VPS (UbiQ-193) is an activity-based probe and inhibitor of DUBs.
crystallizationpull downpurificationwestern blotMSNMRphenotypic protein profilingDUB activity profilingcompound library screeningtarget inhibitor profiling -
His6-Ahx-Ahx-SUMO2-VPS
UbiQ code: UbiQ-237His6-Ahx-Ahx-SUMO2-VPS (UbiQ-237) is an activity-based probe for SUMO proteases.
crystallizationpull downpurificationwestern blotMSNMRphenotypic protein profilingDUB activity profilingcompound library screeningtarget inhibitor profiling -
Ac-ISG15prox-VPS
UbiQ code: UbiQ-262UbiQ-262 is an activity-based probe for and inhibitor of ISG15 proteases.
crystallizationpull downpurificationwestern blotMSNMRphenotypic protein profilingDUB activity profilingcompound library screeningtarget inhibitor profiling -
His10-SUMO3-Dha
UbiQ code: UbiQ-124His10-SUMO3-Dha is a new and first of its kind activity based probe for the SUMO ligation pathway.
crystallizationwestern blot -
Biotin-K63 diUb-VME
UbiQ code: UbiQ-115UbiQ-115 (Biotin-Ahx-K63 diUbiquitin VME) probe is a potent, irreversible and specific inhibitor of deubiquitylating enzymes (DUBs) based on K63 linked diUb. Here Lys63 has been replaced by a diaminobutyric acid residue equipped with a VME type warhead3.
crystallizationpull downpurificationwestern blotphenotypic protein profiling -
Ub-VS
UbiQ code: UbiQ-108Ubiquitin-Viny Sulfone is an inhibitor of deubiquitylating enzymes (DUBs).
crystallizationpurificationwestern blotphenotypic protein profilingDUB activity profiling -
Biotin-Ahx-SUMO2-Dha
UbiQ code: UbiQ-159Biotin-Ahx-SUMO2-Dha (Dha= dehydroalanine) is a new and first of its kind activity based probe for the SUMO ligation pathway. [
crystallizationpull downpurificationwestern blotphenotypic protein profilingtarget inhibitor profiling -
K63 diUb PA
UbiQ code: UbiQ-114Potent, irreversible and specific inhibitor based on K63 linked diUb that targets the S2 pocket of deubiquitinating enzymes (DUBs). The C-terminus of the proximal Ub is equiped with the propargylamide (PA) electrophile and the isopeptide bond between the two Ub proteins is replaced by a DUB resistant mimic.
crystallizationpull downpurificationwestern blotphenotypic protein profiling