reagents

UbiQ-L02 is a panel of the following 10 deubiquitylating enzyme (DUB) activity based probes (ABPs).

UbiQ-L04 contains seven deubiquitylating enzymes (DUB) activity-based probes based on K6, 11, 27, 29, 33, 48, and 63 linked diUb.

UbiQ-158 is an activity-based probe for E1 activating enzymes and E2 conjugating enzymes that are involved in autophagy (e.g. ATG7 and ATG3, respectively). The N-terminus is labeled with an His6 affinity tag and a 3C protease cleavage site (QG), Cys17 has been mutated to a Ser residue (S).

Biotin-Ahx-SUMO2-VME is an activity-based probe for SUMO proteases.

Biotin-ANP-Ub-PA (UbiQ-077) is a newly developed potent and specific inhibitor of deubiquitylating enzymes (DUBs). It is labeled on the N-terminus with biotin and contains the UV cleavable 3-amino-3-(2-nitrophenyl)propanoic acid (ANP) linker, allowing for UV mediated cleavage of the biotin tag.

Ubiquitin-propargylamide is a newly developed potent and specific inhibitor of deubiquitylating enzymes (DUBs).

Ub-VME is a potent, irreversible and specific inhibitor of deubiquitylating enzymes (DUBs),

HA-Ahx-Ahx-Ub-VPS (UbiQ-193) is an activity-based probe and inhibitor of DUBs.

His6-Ahx-Ahx-SUMO2-VPS (UbiQ-237) is an activity-based probe for SUMO proteases.

UbiQ-262 is an activity-based probe for and inhibitor of ISG15 proteases

His10-SUMO3-Dha is a new and first of its kind activity based probe for the SUMO ligation pathway.

UbiQ-115 (Biotin-Ahx-K63 diUbiquitin VME) probe is a potent, irreversible and specific inhibitor of deubiquitylating enzymes (DUBs) based on K63 linked diUb. Here Lys63 has been replaced by a diaminobutyric acid residue equipped with a VME type warhead3.

Ubiquitin-Viny Sulfone is an inhibitor of deubiquitylating enzymes (DUBs).

Biotin-Ahx-SUMO2-Dha (Dha= dehydroalanine) is a new and first of its kind activity based probe for the SUMO ligation pathway. [

Potent, irreversible and specific inhibitor based on K63 linked diUb that targets the S2 pocket of deubiquitinating enzymes (DUBs). The C-terminus of the proximal Ub is equiped with the propargylamide (PA) electrophile and the isopeptide bond between the two Ub proteins is replaced by a DUB resistant mimic.