reagents

UbiQ-158 is an activity-based probe for E1 activating enzymes and E2 conjugating enzymes that are involved in autophagy (e.g. ATG7 and ATG3, respectively). The N-terminus is labeled with an His6 affinity tag and a 3C protease cleavage site (QG), Cys17 has been mutated to a Ser residue (S).

SUMO3-Dha is a new and first of its kind activity based probe for the SUMO ligation pathway.

Ub-VME is a potent, irreversible and specific inhibitor of deubiquitylating enzymes (DUBs),

SUMO1-Dha is a new and first of its kind activity based probe for the SUMO ligation pathway.

human SUMO2 sequence (C48S) which is functionalized on the N-terminus with a 5-carboxyrhodamine110 dye.

His10-SUMO3-Dha is a new and first of its kind activity based probe for the SUMO ligation pathway.

SUMO2-Dha is a new and first of its kind activity based probe for the SUMO ligation pathway.

UbiQ-154 is a new crosslinking reagent based on ubiquitin in which Leu73 is replaced by the photoreactive analog photoleucine (photoLeu).

Biotin-Ahx-SUMO2-Dha (Dha= dehydroalanine) is a new and first of its kind activity based probe for the SUMO ligation pathway. [

Ubiquitin(-like) (e.g. Ub, Nedd8, SUMO, etc.) assay reagents with various modifications (unnatural amino acids, peptides, tags, etc.)

Biotin-Ahx-Ub-Dha is a new activity based probe for Ub E1, E2 and (HECT/RBR) E3 ligases including a biotin tag.

5-carboxyRh110-Ub-Dha is a new activity based probe for Ub E1, E2 and (HECT/RBR) E3 ligases.

Cy5-Ub-Dha is a new activity based probe for Ub E1, E2 and (HECT/RBR) E3 ligases. The N-terminus of UbiQ-104 is labeled with a Cy5 tag.

UbiQ-101 is a new activity based probe for Ub E1, E2 and (HECT/RBR) E3 ligases.

UbiQ-103 is a new activity based probe for Ub E1, E2 and (HECT/RBR) E3 ligases. The N-terminus of UbiQ-103 is labeled with an His6 tag; two aminohexanoic acid (Ahx) linkers are used to create extra space between the His6 and Ub protein for efficient access of His6 binding entities.