reagents

Ubiquitin-propargylamide is a newly developed potent and specific inhibitor of deubiquitylating enzymes (DUBs).

UbiQ-115 (Biotin-Ahx-K63 diUbiquitin VME) probe is a potent, irreversible and specific inhibitor of deubiquitylating enzymes (DUBs) based on K63 linked diUb. Here Lys63 has been replaced by a diaminobutyric acid residue equipped with a VME type warhead3.

UbiQ-174 consists of the ubiquitin protein (Ub) in which Lys6 has been mutated to a cysteine residue.

UbiQ-180 consists of the ubiquitin protein (Ub) in which Lys63 has been mutated to a cysteine residue.

Biotin-Ahx-Ub-VS (UbiQ-188) is a potent and specific inhibitor of deubiquitinating enzymes (DUBs) based on a C-terminal electrophilic vinyl sulfone (VS) group.

UbiQ-023 is a fluorescence polarization HTS control reagent that can be used to make a concentration calibration curve and determine the grating factor (G).

UbiQ-177 consists of the ubiquitin protein (Ub) in which Lys29 has been mutated to a cysteine residue.

Ub-VME is a potent, irreversible and specific inhibitor of deubiquitylating enzymes (DUBs),

Potent, irreversible and specific inhibitor based on K63 linked diUb that targets the S2 pocket of deubiquitinating enzymes (DUBs). The C-terminus of the proximal Ub is equiped with the propargylamide (PA) electrophile and the isopeptide bond between the two Ub proteins is replaced by a DUB resistant mimic.

UbiQ-041 (K382 Ub-p53(368-389)-FP) is a class II fluorescence polarization HTS reagent based on the C-terminal peptide sequence 368 – 389 of p53, which contains various lysine residues that are (mono)ubiquitinated.

UbiQ-175 consists of the ubiquitin protein (Ub) in which Lys11 has been mutated to a cysteine residue.

UbiQ-154 is a new crosslinking reagent based on ubiquitin in which Leu73 is replaced by the photoreactive analog photoleucine (photoLeu).

HA-Ahx-Ahx-Ub-VS (UbiQ-187) is a potent and specific inhibitor of deubiquitinating enzymes (DUBs) based on a C-terminal electrophilic vinyl sulfone (VS) group.

Biotin-ANP-Ub-PA (UbiQ-077) is a newly developed potent and specific inhibitor of deubiquitylating enzymes (DUBs). It is labeled on the N-terminus with biotin and contains the UV cleavable 3-amino-3-(2-nitrophenyl)propanoic acid (ANP) linker, allowing for UV mediated cleavage of the biotin tag.

UbiQ-178 consists of the ubiquitin protein (Ub) in which Lys33 has been mutated to a cysteine residue.