reagents
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Ub photoLeu73
UbiQ code: UbiQ-154UbiQ-154 is a new crosslinking reagent based on ubiquitin in which Leu73 is replaced by the photoreactive analog photoleucine (photoLeu).
crystallizationpull downwestern blotMS -
HA-Ahx-Ahx-Ub-VS
UbiQ code: UbiQ-187HA-Ahx-Ahx-Ub-VS (UbiQ-187) is a potent and specific inhibitor of deubiquitinating enzymes (DUBs) based on a C-terminal electrophilic vinyl sulfone (VS) group.
crystallizationpurificationwestern blotphenotypic protein profilingDUB activity profiling -
Biotin-ANP-Ub-PA
UbiQ code: UbiQ-077Biotin-ANP-Ub-PA (UbiQ-077) is a newly developed potent and specific inhibitor of deubiquitylating enzymes (DUBs). It is labeled on the N-terminus with biotin and contains the UV cleavable 3-amino-3-(2-nitrophenyl)propanoic acid (ANP) linker, allowing for UV mediated cleavage of the biotin tag.
crystallizationpull downpurificationwestern blotphenotypic protein profilingDUB activity profilingtarget inhibitor profiling -
Ub K33C
UbiQ code: UbiQ-178UbiQ-178 consists of the ubiquitin protein (Ub) in which Lys33 has been mutated to a cysteine residue.
DUB activity profilingcompound library screeningtarget inhibitor profiling -
Ub K48C
UbiQ code: UbiQ-179UbiQ-179 consists of the ubiquitin protein (Ub) in which Lys48 has been mutated to a cysteine residue.
DUB activity profilingcompound library screeningtarget inhibitor profiling -
K382 Ub-p53(357-389)-FP
UbiQ code: UbiQ-042UbiQ-041 (K382 Ub-p53(368-389)-FP) is a class II fluorescence polarization HTS reagent based on the C-terminal peptide sequence 368 – 389 of p53, which contains various lysine residues that are (mono)ubiquitinated.
DUB activity profilingcompound library screening -
Ub K27C
UbiQ code: UbiQ-176UbiQ-176 consists of the ubiquitin protein (Ub) in which Lys27 has been mutated to a cysteine residue.
DUB activity profilingcompound library screeningtarget inhibitor profiling -
Ub-PA
UbiQ code: UbiQ-057UbiQ-057 (Ub-PA) is an activity-based probe for deubiquitinating enzymes (DUBs) and contains propargyl amide (PA) as an electrophile.
crystallizationpull downpurificationwestern blotMSNMRphenotypic protein profiling -
Biotin-K63 diUb-VME
UbiQ code: UbiQ-115UbiQ-115 (Biotin-Ahx-K63 diUbiquitin VME) probe is a potent, irreversible and specific inhibitor of deubiquitylating enzymes (DUBs) based on K63 linked diUb. Here Lys63 has been replaced by a diaminobutyric acid residue equipped with a VME type warhead3.
crystallizationpull downpurificationwestern blotphenotypic protein profiling -
Ub K6C
UbiQ code: UbiQ-174UbiQ-174 consists of the ubiquitin protein (Ub) in which Lys6 has been mutated to a cysteine residue.
DUB activity profilingcompound library screeningtarget inhibitor profiling -
Ub K63C
UbiQ code: UbiQ-180UbiQ-180 consists of the ubiquitin protein (Ub) in which Lys63 has been mutated to a cysteine residue.
DUB activity profilingcompound library screeningtarget inhibitor profiling -
Biotin-Ahx-Ub-VS
UbiQ code: UbiQ-188Biotin-Ahx-Ub-VS (UbiQ-188) is a potent and specific inhibitor of deubiquitinating enzymes (DUBs) based on a C-terminal electrophilic vinyl sulfone (VS) group.
crystallizationpurificationwestern blotphenotypic protein profilingDUB activity profiling -
5-TAMRA-Lys-Gly-OH
UbiQ code: UbiQ-023UbiQ-023 is a fluorescence polarization HTS control reagent that can be used to make a concentration calibration curve and determine the grating factor (G).
compound library screening -
Ub K29C
UbiQ code: UbiQ-177UbiQ-177 consists of the ubiquitin protein (Ub) in which Lys29 has been mutated to a cysteine residue.
DUB activity profilingcompound library screeningtarget inhibitor profiling -
Ub-VME
UbiQ code: UbiQ-005Ub-VME (UbiQ-005) is a potent, irreversible and specific inhibitor of deubiquitinating enzymes (DUBs).
crystallizationpull downpurificationwestern blotMSNMRphenotypic protein profilingDUB activity profilingcompound library screeningtarget inhibitor profiling
