reagents

Ub-VME is a potent, irreversible and specific inhibitor of deubiquitylating enzymes (DUBs),

UbiQ-287 is a fluorescence polarization assay reagent for deISGylating enzymes which is based on a 5-carboxytetramethylrhodamine (TAMRA, exc 550 nm, emi 590 nm) modified Lys-Gly sequence that is linked to human ISG15 via a native isopeptide bond.

HA-Ahx-Ahx-Ub-VPS (UbiQ-193) is an activity-based probe and inhibitor of DUBs.

Biotin-Ahx-SUMO2-VME is an activity-based probe for SUMO proteases.

His6-Ahx-Ahx-SUMO2-VPS (UbiQ-237) is an activity-based probe for SUMO proteases.

Nedd8-FP (UbiQ-019) is a fluorescence polarization assay reagent which is based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to Nedd8 via a native isopeptide bond with the lysine side-chain.

UbiQ-073 is a fluorescence polarization assay reagent for deISGylating enzymes which is based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to (mouse) ISG15 via a native isopeptide bond with the lysine side-chain.

Ac-ISG15prox-Rh110MP (UbiQ-127) is a quenched, fluorescent substrate for ISG15 proteases based on the proximal part of (mouse) ISG15 (aa 77-155).

SUMO1-FP (UbiQ-020) is a fluorescence polarization assay reagent for de-SUMOylating enzyme, based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to (6His tagged) SUMO-1 via a native isopeptide bond with the lysine side-chain.

SUMO2-FP (UbiQ-021) is a fluorescence polarization assay reagent for de-SUMOylating enzyme, based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to (6His tagged) SUMO-2 via a native isopeptide bond with the lysine side-chain.

Class II fluorescence polarization HTS reagent based on the cullin-1 (711-730) polypeptide

Ubiquitin(-like) (e.g. Ub, Nedd8, SUMO, etc.) assay reagents with various modifications (unnatural amino acids, peptides, tags, etc.)

Custom ubiquitin(-like) probes with various C-terminal warheads (e.g. VME= vinylmethyl ester, PA = propagylamide, VS= vinyl sulfone, Dha= dehydroalanine) and N-terminal tags (e.g. TAMRA, Cy5, biotin).

Our UbiQ-PEP™ service provides isopeptide linked Ub-polypeptide conjugates with a range of modifications to meet your research needs.