reagents
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Ub-VME
UbiQ code: UbiQ-005Ub-VME is a potent, irreversible and specific inhibitor of deubiquitylating enzymes (DUBs),
crystallizationpull downpurificationwestern blotMSNMRphenotypic protein profilingDUB activity profilingcompound library screeningtarget inhibitor profiling -
hISG15-FP
UbiQ code: UbiQ-287UbiQ-287 is a fluorescence polarization assay reagent for deISGylating enzymes which is based on a 5-carboxytetramethylrhodamine (TAMRA, exc 550 nm, emi 590 nm) modified Lys-Gly sequence that is linked to human ISG15 via a native isopeptide bond.
DUB activity profilingcompound library screening -
HA-Ahx-Ahx-Ub-VPS
UbiQ code: UbiQ-193HA-Ahx-Ahx-Ub-VPS (UbiQ-193) is an activity-based probe and inhibitor of DUBs.
crystallizationpull downpurificationwestern blotMSNMRphenotypic protein profilingDUB activity profilingcompound library screeningtarget inhibitor profiling -
Biotin-Ahx-SUMO2-VME
UbiQ code: UbiQ-156Biotin-Ahx-SUMO2-VME is an activity-based probe for SUMO proteases.
crystallizationpull downpurificationwestern blotphenotypic protein profilingDUB activity profilingtarget inhibitor profiling -
His6-Ahx-Ahx-SUMO2-VPS
UbiQ code: UbiQ-237His6-Ahx-Ahx-SUMO2-VPS (UbiQ-237) is an activity-based probe for SUMO proteases.
crystallizationpull downpurificationwestern blotMSNMRphenotypic protein profilingDUB activity profilingcompound library screeningtarget inhibitor profiling -
SUMO2-FP
UbiQ code: UbiQ-021SUMO2-FP (UbiQ-021) is a fluorescence polarization assay reagent for de-SUMOylating enzyme, based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to (6His tagged) SUMO-2 via a native isopeptide bond with the lysine side-chain.
DUB activity profilingcompound library screening -
NEDD8-FP
UbiQ code: UbiQ-019Nedd8-FP (UbiQ-019) is a fluorescence polarization assay reagent which is based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to Nedd8 via a native isopeptide bond with the lysine side-chain.
DUB activity profilingcompound library screening -
ISG15-FP
UbiQ code: UbiQ-073UbiQ-073 is a fluorescence polarization assay reagent for deISGylating enzymes which is based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to (mouse) ISG15 via a native isopeptide bond with the lysine side-chain.
DUB activity profilingcompound library screening -
SUMO1-FP
UbiQ code: UbiQ-020SUMO1-FP (UbiQ-020) is a fluorescence polarization assay reagent for de-SUMOylating enzyme, based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to (6His tagged) SUMO-1 via a native isopeptide bond with the lysine side-chain.
DUB activity profilingcompound library screening -
K720 Nedd8-cullin1 (711-730)-FP
UbiQ code: UbiQ-080Class II fluorescence polarization HTS reagent based on the cullin-1 (711-730) polypeptide
DUB activity profilingcompound library screening -
custom assay reagents
UbiQ code: UbiQ-C01 ref POUbiquitin(-like) (e.g. Ub, Nedd8, SUMO, etc.) assay reagents with various modifications (unnatural amino acids, peptides, tags, etc.)
crystallizationpull downpurificationwestern blotMSNMRphenotypic protein profilingDUB activity profilingcompound library screeningtarget inhibitor profiling -
custom probes
UbiQ code: UbiQ-C02Custom ubiquitin(-like) probes with various C-terminal warheads (e.g. VME= vinylmethyl ester, PA = propagylamide, VS= vinyl sulfone, Dha= dehydroalanine) and N-terminal tags (e.g. TAMRA, Cy5, biotin).
crystallizationpull downpurificationwestern blotMSNMRphenotypic protein profilingDUB activity profilingcompound library screeningtarget inhibitor profiling -
custom peptides
UbiQ code: UbiQ-C03Our UbiQ-PEP™ service provides isopeptide linked Ub-polypeptide conjugates with a range of modifications to meet your research needs.
crystallizationpull downpurificationwestern blotMSNMRphenotypic protein profilingDUB activity profilingcompound library screeningtarget inhibitor profiling
