Biotin-Ahx-Ub(1-75)-Dha-Ub

Biotin-Ahx-Ub(1-75)-Dha-Ub

details

UbiQ-121 is an activity based probe based on linear diubiquitin which targets the deubiquitinating enzyme (DUB) OTULIN. In UbiQ-121, the Gly76-Met1 dipeptide of linear diUb is replaced by a dehydroalanine-Met (Dha-Met) dipeptide. The N-terminus of the distal Ub is labeled with biotin; an aminohexanoic acid (Ahx) linker is used to create extra space for efficient access of biotin binding entities. UbiQ-121 has been prepared by total chemical synthesis and is therefore well-defined in terms of biotinylation site. UbiQ-121 reacts with OTULIN in a covalent manner without being cleaved into monoUb, indicating correct positioning of the Dha electrophile for reacting with the nucleophilic active site cysteine of OTULIN. The DUB activity based probe can be used for activity profiling experiments and structural studies. Please note that the native distance between the proximal and distal Ub is preserved.

Product Attachments

View FileUbiQ-121 Productsheet B01062015-001
crystallizationpull downpurificationwestern blotMSNMRphenotypic protein profiling

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mg
name Biotin-Ahx-Ub(1-75)-Dha-Ub
product code UbiQ-121
Applications crystallization, pull down, purification, western blot, MS, NMR, phenotypic protein profiling
target DUB
source human sequence, synthetic
shipping lyophilized powder
purity ≥95% by SDS-PAGE
molecular weight 17.46 kDa
storage upon arrival powder at −20°C, solution at −80°C.
sample preparation For detailed sample preparation see product sheet.
regulatory statement For laboratory research use only, not for use in humans.
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    https://www.ncbi.nlm.nih.gov/pubmed/28919039

  • UbiQ-Probes crystallization overview download this pdf
  • UbiQ_DUB Probes factsheet download this pdf
  • UbiQ's drug discovery expertise overview

     

    download this pdf