UbiQ-002

Ub-Rh110Gly

a quenched, fluorescent substrate for deubiquitinating enzymes

code UbiQ-002 Category activity assay reagents Tags assay, compound library screening, DUB activity assay, fluorescence polarization, ubiquitin

product UbiQ-002 Category activity assay reagents Tags assay, compound library screening, DUB activity assay, fluorescence polarization, ubiquitin

Additional information

Weight	0.05 kg
aliquot size	100 µg
bulk	1 mg, 5 mg, 10 mg, 20 mg
Applications	DUB activity profiling, Compound library screening
target	DUB
shipping	lyophilized powder
purity	≥95%
molecular weight	8.93 kDa
storage	Upon arrival, powder at −20°C; solution at −80°C. Protect from light and avoid multiple freeze/thaw cycles.
sample preparation	For detailed sample preparation see product sheet.
regulatory statement	For laboratory research use only, not for use in humans.

€375.00

Description
Additional information
references
ubiquitin rhodamine

Description

Ubiquitin-Rhodamine110Gly (UbiQ-002) is a quenched fluorescent substrate for deubiquitinating enzymes. Cleavage of the amide bond between the C-terminal glycine of ubiquitin and rhodamine110Gly (Rh110Gly) results in an increase in fluorescence at 535 nm (Exc. 485 nm).

Additional information

Weight	0.05 kg
aliquot size	100 µg
bulk	1 mg, 5 mg, 10 mg, 20 mg
Applications	DUB activity profiling, Compound library screening
target	DUB
shipping	lyophilized powder
purity	≥95%
molecular weight	8.93 kDa
storage	Upon arrival, powder at −20°C; solution at −80°C. Protect from light and avoid multiple freeze/thaw cycles.
sample preparation	For detailed sample preparation see product sheet.
regulatory statement	For laboratory research use only, not for use in humans.

Tirat, A., et al. Synthesis and characterization of fluorescent ubiquitin derivatives as highly sensitive substrates for the deubiquitinating enzymes UCH-L3 and USP-2. Analytical Biochem 2005, 343, 244. http://www.ncbi.nlm.nih.gov/pubmed/15963938

Hassiepin, U., et al. A sensitive fluorescence intensity assay for deubiquitinating proteases using ubiquitin-rhodamine 110-glycine as substrate. Analytical Biochem 2007, 371, 201. http://www.ncbi.nlm.nih.gov/pubmed/17869210

Ubiquitin rhodamine (also known as ubiquitin-rhodamine110Gly, Ub-Rh110Gly, UbiQ-002) is a quenched fluorogenic substrate for deubiquitinating enzymes (DUBs). It is based on ubiquitin that is functionalised with a C-terminal rhodamine110Gly (Rh110Gly). DUB cleavage of the amide bond between the C-terminal Gly and Rh110 releases the fluorescent Rh110Gly dye (exc/emi= 485/535 nm). Ubiquitin rhodamine110 has been proven in many DUB high-throughput screening (HTS) campaigns.

Next to ubiquitin rhodamine110 (UbiQ-002), we developed ubiquitin rhodamine110-morpholinecarbonyl (Ub-Rh110MP, UbiQ-126). Ub-Rh110MP is also a quenched, fluorescent substrate for DUBs, with cleavage resulting in the release of the highly fluorescent Rh110-morpholinecarbonyl (Rh110MP, exc/emi= 492/525 nm). Since Rh110MP exhibits a much higher fluorescence intensity than most Rh110X-based dyes, UbiQ-126 exhibits a higher signal-to-background ratio. Overall, Ub-Rh110MP offers the excellent properties of Ub-Rh110X substrates, but with increased fluorescence intensity after proteolytic cleavage.

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