Ub-Rh110Gly
a quenched, fluorescent substrate for ubiquitin proteases
Additional information
| Weight | 0.05 kg |
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| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
€300.00
- Description
- Additional information
- references
Description
Ubiquitin-Rhodamine110Gly (UbiQ-002) is a quenched fluorescent substrate for ubiquitin proteases. Cleavage of the amide bond between the C-terminal glycine of ubiquitin and rhodamine110Gly (Rh110Gly) results in an increase in fluorescence at 535 nm (Exc. 485 nm).
Additional information
| Weight | 0.05 kg |
|---|---|
| aliquot size | |
| bulk | |
| Applications | |
| target | |
| shipping | |
| purity | |
| molecular weight | |
| storage | |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
Tirat, A., et al. Synthesis and characterization of fluorescent ubiquitin derivatives as highly sensitive substrates for the deubiquitinating enzymes UCH-L3 and USP-2. Anal. Biochem. 343, 244-255 (2005). http://www.ncbi.nlm.nih.gov/pubmed/15963938
Hassiepin, U., et al. A sensitive fluorescence intensity assay for deubiquitinating proteases using ubiquitin-rhodamine 110-glycine as substrate. Anal. Biochem. 371, 201-207 (2007). http://www.ncbi.nlm.nih.gov/pubmed/17869210