SUMO1-FP (UbiQ-020) is a fluorescence polarization assay reagent for de-SUMOylating enzymes which is based on a 5-carboxytetramethylrhodamine (TAMRA, exc 550 nm, emi 590 nm) modified Lys-Gly sequence that is linked to (6His tagged) SUMO-1 via a native isopeptide bond with the lysine side-chain.

A typical substrate concentration is 100 nM. Effective concentrations of deSUMOylating enzymes can range from 0.01-10 nM but depend on specific assay conditions and method of detection.

UbiQ-020 is effectively processed by SENP1.

Product Attachments

View FileUbiQ-020 Productsheet B29072013-001
DUB activity profilingcompound library screening

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name SUMO1-FP
product code UbiQ-020
Applications DUB activity profiling, compound library screening
target Ubl proteases
source human sequence, semi-synthetic
shipping 100 uM solution in milliQ/10 vol% DMSO
purity >95%
molecular weight 11.58 kDa
storage Solution at −80°C. Protect from light. Please avoid multiple freeze/thaw cycles.
sample preparation upon arrival, powder at −20°C, solution at −80°C. Protect from light and avoid multiple freeze/thaw cycles.
regulatory statement For laboratory research use only, not for use in humans.
  • A. Tirat et al.

    Tirat, A., et al. Synthesis and characterization of fluorescent ubiquitin derivatives as highly sensitive substrates for the deubiquitinating enzymes UCH-L3 and USP-2. Anal. Biochem. 343, 244-255 (2005).

  • Huang et al.

    Huang, X. & Aulabaugh A. Application of fluorescence polarization in HTS assays. Methods in Molecular Biology 565, 127-143 (2009).

  • Levine et al.

    Levine, L.M., et al. Measurement of specific protease activity utilizing fluorescence polarization. Anal. Biochem. 247, 83-88 (1997).

  • Geurink and El Oualid et al.

    Geurink, P.P., et al. A general chemical ligation approach towards isopeptide-linked ubiquitin and ubiquitin-like assay reagents. ChemBiochem 13, 293-297 (2012).

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