UbiQ-035 (HA-Ahx-Ahx-Ub-VME) is a potent, irreversible and specific inhibitor of deubiquitylating enzymes (DUBs) that is prepared by chemical synthesis. It is N-terminally tagged with an HA-tag. The HA peptide sequence (YPYDVPDYA) is derived from the influenza hemagglutinin protein and allows for the sensitive identification or purification of DUBs since it is specifically recognized by anti-HA antibodies and/or anti-HA-agarose. The HA tag is separated from the Ub N-terminus by two aminohexanoic acid (Ahx) linkers for efficient recognition of the tag.

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View FileUbiQ-035 Productsheet B01042014-001
crystallizationpull downpurificationwestern blotphenotypic protein profiling

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name HA-Ahx-Ahx-Ub-VME
product code UbiQ-035
Applications crystallization, pull down, purification, western blot, phenotypic protein profiling
target DUB
source human sequence, synthetic
shipping lyophilized powder
purity ≥95% by RP-HPLC
molecular weight 9.91 kDa
storage upon arrival powder at −20°C; solution at −80°C. Avoid multiple freeze/thaw cycles.
sample preparation For detailed sample preparation see product sheet.
regulatory statement For laboratory research use only, not for use in humans.
  • F. El Oualid et al.

    El Oualid, F., et al. Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin. Angewandte Chemie Int. Ed. 49, 10149-10153 (2010).


  • Borodovsky et al.

    Borodovsky, A., et al. A novel active site-directed probe specific for deubiquitylating enzymes reveals proteasome association of USP14. EMBO J. 20, 5187-5196 (2001).


  • Borodovsky et al.

    Borodovsky, A., et al. Chemistry-based functional proteomics reveals novel members of the deubiquitinating enzyme family. Chem. Biol. 9, 1149-1159 (2002). 


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