K382 Ub-p53(368-389)-FP

K382 Ub-p53(368-389)-FP

details

UbiQ-041 (K382 Ub-p53(368-389)-FP) is a class II fluorescence polarization HTS reagent based on the C-terminal peptide sequence 368 – 389 of p53, which contains various lysine residues that are (mono)ubiquitinated. The peptide is modified on the N-terminus with a 5-carboxytetramethylrhodamine and conjugated at Lys382 to Ub via a native isopeptide bond. See Geurink er al. 2012 (open access) for full experimental and analytical details.

Product Attachments

View FileUbiQ-041 Productsheet B09082012-001
DUB activity profilingcompound library screening

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mg
name K382 Ub-p53(368-389)-FP
product code UbiQ-041
Applications DUB activity profiling, compound library screening
target DUB
source human, synthetic
shipping lyophilized powder (purple)
purity ≥95% by RP-HPLC and SDS-PAGE
molecular weight 11518 Da by MS (calc 11523 Da)
storage −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles.
sample preparation For detailed sample preparation see product sheet.
regulatory statement For laboratory research use only, not for use in humans.
    • Geurink et al.

      A general chemical ligation approach towards isopeptide-linked ubiquitin and ubiquitin-like assay reagents. 

      Chembiochem. 13 (2): 293-297. (2012), DOI:10.1002/cbic.201100706.

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