details
UbiQ-041 (K382 Ub-p53(368-389)-FP) is a class II fluorescence polarization HTS reagent based on the C-terminal peptide sequence 368 – 389 of p53, which contains various lysine residues that are (mono)ubiquitinated. The peptide is modified on the N-terminus with a 5-carboxytetramethylrhodamine and conjugated at Lys382 to Ub via a native isopeptide bond. See Geurink er al. 2012 (open access) for full experimental and analytical details.