K382 Ub-p53(357-389)-FP

K382 Ub-p53(357-389)-FP

details

UbiQ-042 (K382 Ub-p53(357-389)-FP) is a class II fluorescence polarization HTS reagent based on the C-terminal peptide sequence 357- 389 of p53, which contains various lysine residues that are (mono)ubiquitinated. The peptide is modified on the N-terminus with a 5-carboxytetramethylrhodamine and conjugated at Lys382 to Ub via a native isopeptide bond. This site was chosen because USP7 (HAUSP), which deubiquitinates p53, was found to exhibit high activity to peptides with an Ub on Lys382 (unpublished data). Furthermore, this class II reagent contains a PGGS motif (aa 359-362) which was found to be important for USP7 binding. (Sheng et al. 2006)

Product Attachments

View FileUbiQ-042 Productsheet B09082012-001
DUB activity profilingcompound library screening

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mg
name K382 Ub-p53(357-389)-FP
product code UbiQ-042
Applications DUB activity profiling, compound library screening
target DUB
source human, synthetic
shipping lyophilized powder (purple)
purity ≥95% by RP-HPLC and SDS-PAGE
molecular weight 12613 Da by MS (calc 12619 Da)
storage −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles.
sample preparation For detailed sample preparation see product sheet.
regulatory statement For laboratory research use only, not for use in humans.
    • Geurink et al.

      A general chemical ligation approach towards isopeptide-linked ubiquitin and ubiquitin-like assay reagents. 

      Chembiochem. 13 (2): 293-297. (2012), DOI:10.1002/cbic.201100706.

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