UbiQ-057 (Ub-PA) is an activity-based probe for deubiquitinating enzymes (DUBs) and contains propargyl amide (PA) as an electrophile. UbiQ-057 can be used for activity profiling experiments and determining DUB inhibitor specificity. It has two unique capabilities: 1) It forms a covalent linkage with (the active site Cys residue of) a DUB that can be cleaved by acid treatment (5% aq. TFA), allowing for proteomic analyses. 2) It targets all three major DUB families: UCH, USP and OTU.

Product Attachments

View FileUbiQ-057 Productsheet B01102012-001
crystallizationpull downpurificationwestern blotMSNMRphenotypic protein profiling

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name Ub-PA
product code UbiQ-057
Applications crystallization, pull down, purification, western blot, MS, NMR, phenotypic protein profiling
target DUB
source human sequence, synthetic
shipping lyphilized powder
purity ≥95% by RP-HPLC
molecular weight 8.55 kDa
storage Powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles.
sample preparation For detailed sample preparation see product sheet.
regulatory statement For laboratory research use only, not for use in humans.
    • Galardy et al.

      Galardy, P., et al. Mechanism-based proteomics tools based on ubiquitin and ubiquitin-like proteins: crystallography, activity profiling, and protease identification. Methods in Enzymology. 399, 120-131 (2005).



    • Ekkebus et al.

      Ekkebus, R., et al. On terminal alkynes that can react with active-site cysteine nucleophiles in proteases. J. Am. Chem. Soc. 135, 2867-2870 (2013). 


    • Sommer et al.

      Sommer, S., et al. Covalent inhibition of SUMO and ubiquitin-specific cysteine proteases by an in situ thiol-alkyne addition. Bioorg. Med. Chem. 21, 2511-2517 (2013).