TAMRA-Ub-PA

fluorescent activity-based probe for deubiquitinating enzymes (DUBs)

productsheet

code UbiQ-058 Category Tags , ,
product UbiQ-058 Category Tags , ,

Additional information

Weight 0.005 kg
aliquot size

Applications

, , , ,

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source

human, synthetic
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purity

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sample preparation

For detailed sample preparation see product sheet.
regulatory statement

€250.00

Description

UbiQ-058 is an activity-based probe for deubiquitinating enzymes (DUBs). It is based on ubiquitin functionalised with a C-terminal propargyl amide (PA) and N-terminal 5-carboxytetramethylrhodamine (TAMRA) dye. It can be used for activity profiling experiments and determining DUB inhibitor specificity.

  • the PA group forms a covalent linkage with (the active site Cys residue of) a DUB that can be cleaved by acid treatment (5% aq. TFA), allowing for proteomic analyses;
  • the PA group targets all three major DUB families: UCH, USP and OTU;
  • the fluorescent label allows detection of DUB labeling by direct in-gel fluorescence. This is a less time-consuming and more sensitive read-out than western-blotting
  • cross-reactivity of antibodies can lead to background labeling, something that is not observed with UbiQ-058

Additional information

Weight 0.005 kg
aliquot size

Applications

, , , ,

target

source

human, synthetic
shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.
regulatory statement

Galardy et al.
Galardy, P., et al. Mechanism-based proteomics tools based on ubiquitin and ubiquitin-like proteins: crystallography, activity profiling, and protease identification. Methods in Enzymology. 399, 120-131 (2005).
http://www.ncbi.nlm.nih.gov/pubmed/16338352

Ekkebus et al.
Ekkebus, R., et al. On terminal alkynes that can react with active-site cysteine nucleophiles in proteases. J. Am. Chem. Soc. 135, 2867-2870 (2013).
http://www.ncbi.nlm.nih.gov/pubmed/23387960