Cy5-Ub-VME
fluorescent activity-based probe for deubiquitinating enzymes (DUBs)
Additional information
| Weight | 0.005 kg |
|---|---|
| aliquot size | |
| Custom Aliquot size | |
| Applications | Crystallization, Pull down, Purification, Western Blot, Phenotypic protein profiling |
| target | |
| source | |
| shipping | |
| purity | |
| molecular weight | |
| storage | Powder at −20°C, solution at −80°C. Protect from light. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
€300.00
- Description
- Additional information
- references
Description
UbiQ-071 is an activity-based probe for deubiquitinating enzymes (DUBs). It is based on ubiquitin functionalised with a C-terminal electrophilic vinyl methyl ester (VME) and N-terminal Cy5 dye. It can be used for activity profiling experiments and determining DUB inhibitor specificity.
- the fluorescent label allows detection of DUB labeling by direct in-gel fluorescence. This is a less time-consuming and more sensitive read-out than western-blotting
- cross-reactivity of antibodies can lead to background labeling, something that is not observed with UbiQ-071
Additional information
| Weight | 0.005 kg |
|---|---|
| aliquot size | |
| Custom Aliquot size | |
| Applications | Crystallization, Pull down, Purification, Western Blot, Phenotypic protein profiling |
| target | |
| source | |
| shipping | |
| purity | |
| molecular weight | |
| storage | Powder at −20°C, solution at −80°C. Protect from light. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
Misaghi, S., et al. Structure of the Ubiquitin Hydrolase UCH-L3 Complexed with a Suicide Substrate. J. Biol. Chem. 280, 1512-1520 (2005).
http://www.ncbi.nlm.nih.gov/pubmed/15531586
de Jong, A., et al. Ubiquitin-based probes prepared by total synthesis to profile the activity of deubiquitinating enzymes. ChemBiochem 13, 2251-2258 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/23011887
Altun, M., et al. Activity-based chemical proteomics accelerates inhibitor development for deubiquitylating enzymes. Chem. Biol. 18, 1401-1412 (2011).
http://www.ncbi.nlm.nih.gov/pubmed/22118674
Wrigley, J.D. et al. Enzymatic characterisation of USP7 deubiquitinating activity and inhibition. Cell Biochem. Biophys. 60, 99-111 (2011)
http://www.ncbi.nlm.nih.gov/pubmed/21468692