Biotin-Ahx-Ub-PA (UbiQ-076) is a newly developed potent and specific inhibitor of deubiquitylating enzymes (DUBs), which is labeled on the N-terminus with biotin. An aminohexanoic acid linker is used to create extra space between the biotin and Ub protein for efficient access of biotin binding entities. This activity probe can be used for activity profiling experiments and determining DUB inhibitor specificity. The C-terminal propargylamide (PA) electrophile has two characteristics: 1. it forms a covalent linkage with (the active site Cys residue of) a DUB that can be cleaved by acid treatment (5% aq. TFA), allowing for proteomic analyses. 2. it targets all three major DUB families: UCH, USP and OTU.

Product Attachments

View FileUbiQ-076 Productsheet B01082013-001
crystallizationpull downpurificationwestern blotMSNMRphenotypic protein profiling

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name Biotin-Ahx-Ub-PA
product code UbiQ-076
Applications crystallization, pull down, purification, western blot, MS, NMR, phenotypic protein profiling
target DUB
source human sequence, synthetic
shipping lyophilized powder
purity ≥95%
molecular weight 8.89 kDa
storage upon arrival, powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles.
sample preparation For detailed sample preparation see product sheet.
regulatory statement For laboratory research use only, not for use in humans.
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