Biotin-ANP-Ub-PA (UbiQ-077) is a newly developed potent and specific inhibitor of deubiquitylating enzymes (DUBs). It is labeled on the N-terminus with biotin and contains the UV cleavable 3-amino-3-(2-nitrophenyl)propanoic acid (ANP) linker, allowing for UV mediated cleavage of the biotin tag.
This activity probe can be used for activity profiling experiments and determining DUB inhibitor specificity. It has two unique capabilities:
1. it forms a covalent linkage with (the active site Cys residue of) a DUB that can be cleaved by acid treatment (5% aq. TFA), allowing for proteomic analyses.
2. it targets all three major DUB families: UCH, USP and OTU.