Biotin-ANP-Ub-PA

Biotin-ANP-Ub-PA

details

Biotin-ANP-Ub-PA (UbiQ-077) is a newly developed potent and specific inhibitor of deubiquitylating enzymes (DUBs). It is labeled on the N-terminus with biotin and contains the UV cleavable 3-amino-3-(2-nitrophenyl)propanoic acid (ANP) linker, allowing for UV mediated cleavage of the biotin tag.

This activity probe can be used for activity profiling experiments and determining DUB inhibitor specificity. It has two unique capabilities:

1. it forms a covalent linkage with (the active site Cys residue of) a DUB that can be cleaved by acid treatment (5% aq. TFA), allowing for proteomic analyses.

2. it targets all three major DUB families: UCH, USP and OTU.

Product Attachments

View FileUbiQ-077 productsheet B01082013-001

Rodenko et al. Nat Prot 2006, 1, 1120

https://www.ncbi.nlm.nih.gov/pubmed/17406393 

 

Ekkebus et al. J. Am. Chem. Soc. 2013, 135, 2867

https://www.ncbi.nlm.nih.gov/pubmed/23387960

 

Sommer et al. Bioorg. Med. Chem. 2013, 21, 2511

https://www.ncbi.nlm.nih.gov/pubmed/23535560 

crystallizationpull downpurificationwestern blotphenotypic protein profilingDUB activity profilingtarget inhibitor profiling

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mg
name Biotin-ANP-Ub-PA
product code UbiQ-077
Applications crystallization, pull down, purification, western blot, phenotypic protein profiling, DUB activity profiling, target inhibitor profiling
target DUB
source human sequence, synthetic
shipping lyophilized powder
purity ≥95% by RP-HPLC
molecular weight 9 kDa
storage upon arrival powder at −20°C; buffered solution at −80°C. Please avoid exposure to light and multiple freeze/thaw cycles.
sample preparation For detailed sample preparation see product sheet.
regulatory statement For laboratory research use only, not for use in humans.