K720 Nedd8-cullin1 (711-730)-FP

K720 Nedd8-cullin1 (711-730)-FP

details

UbiQ-080 is a class II fluorescence polarization HTS reagent based on the cullin-1 (711-730) polypeptide. The peptide is modified on the N-terminus with a 5-carboxytetramethylrhodamine and mononeddylated at K720 via a native isopeptide bond. It can be used as a substrate for Nedd8 proteases. This product is formed by chemical ligation. See reference 4 in the product sheet (open access) for full experimental assay details.

Product Attachments

View FileUbiQ-080 Productsheet B24072012-001
DUB activity profilingcompound library screening

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mg
name K720 Nedd8-cullin1 (711-730)-FP
product code UbiQ-080
Applications DUB activity profiling, compound library screening
target Ubl proteases
source human, synthetic
shipping lyophilized powder
purity ≥95% by SDS-PAGE analysis
molecular weight 11.365 Da
storage Powder at −20°C, solution at −80°C. Please avoid multiple freeze/thaw cycles.
sample preparation For detailed sample preparation see product sheet.
regulatory statement For laboratory research use only, not for use in humans.
  • A. Tirat et al.

    Tirat, A., et al. Synthesis and characterization of fluorescent ubiquitin derivatives as highly sensitive substrates for the deubiquitinating enzymes UCH-L3 and USP-2. Anal. Biochem. 343, 244-255 (2005).

    http://www.ncbi.nlm.nih.gov/pubmed/15963938

  • Huang et al.

    Huang, X. & Aulabaugh A. Application of fluorescence polarization in HTS assays. Methods in Molecular Biology 565, 127-143 (2009).

    http://www.ncbi.nlm.nih.gov/pubmed/19551360

  • Levine et al.

    Levine, L.M., et al. Measurement of specific protease activity utilizing fluorescence polarization. Anal. Biochem. 247, 83-88 (1997). 

    http://www.ncbi.nlm.nih.gov/pubmed/9126375

  • Geurink and El Oualid et al.

    Geurink, P.P., et al. A general chemical ligation approach towards isopeptide-linked ubiquitin and ubiquitin-like assay reagents. ChemBiochem 13, 293-297 (2012).  

    http://www.ncbi.nlm.nih.gov/pubmed/22213387

  • Faesen et al.

    Faesen, A.C., et al. The Differential Modulation of USP Activity by Internal Regulatory Domains, Interactors and Eight Ubiquitin Chain Types. Chem. Biol. 18, 1550-1561 (2011). 

    http://www.ncbi.nlm.nih.gov/pubmed/22195557 

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