Biotin-Ahx-Ub pSer 65

Biotin-Ahx-Ub pSer 65

details

UbiQ-091 is ubiquitin that is phosphorylated on Ser65, a ubiquitin variant which has been shown to activate Parkin E3 ligase mediated ubiquitination. It is labeled on the N-terminus with biotin; an aminohexanoic acid (Ahx) linker is used to create extra space between the biotin and Ub protein for efficient access of biotin binding entities. It has been made by total chemical synthesis and is therefore well-defined in terms of biotin and pSer site and incorporation efficiency (100%).

Product Attachments

View FileUbiQ-091 Productsheet B01102014-001

Kane et al. J Cell Biol 2014, 205, 143-153. https://www.ncbi.nlm.nih.gov/pubmed/24751536

Kazlauskaite et al. Biochem J 2014, 460, 127-139. https://www.ncbi.nlm.nih.gov/pubmed/24660806

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pull downpurificationphenotypic protein profilingcompound library screening

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mg
name Biotin-Ahx-Ub pSer 65
product code UbiQ-091
Applications pull down, purification, phenotypic protein profiling, compound library screening
target n/a
source human, synthetic
shipping lyophilized powder
purity ≥95% by RP-HPLC and SDS-PAGE analysis
molecular weight 8.98 kDa
storage Powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles.
sample preparation For detailed sample preparation see product sheet.
regulatory statement For laboratory research use only, not for use in humans.