Ub-Dha

Ub-Dha

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UbiQ-101 (Ub-Dha, Dha= dehydroalanine) is a new and first of its kind activity based probe for Ub E1, E2 and (HECT/RBR) E3 ligases [Mulder et al. 2016]. It is based on the Ub sequence in which the C-terminal Gly76 has been replaced by a dehydroalanine residue. It is processed in a native manner by Ub E1, E2 and (HECT/RBR) E3 ligases and during this process it forms an electrophilic intermediate that can react with the active site Cys residue of the E1, E2 and (HECT/RBR) E3 enzyme, thereby creating a covalent bond (see figure).

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View FileUbiQ-101 Productsheet B01042015-001
crystallizationwestern blot

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mg
name Ub-Dha
product code UbiQ-101
Applications crystallization, western blot
target E1/E2/E3
source synthetic, human sequence
shipping lyophilized powder
purity ≥95% by RP-HPLC
molecular weight 8.57 kDa
storage Powder at −20°C; solution at −80°C. Protect from light. Please avoid multiple freeze/thaw cycles.
sample preparation For detailed sample preparation see product sheet.
regulatory statement For laboratory research use only, not for use in humans.
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  • Mulder, A., et al.

    Mulder, A., et al. A cascading activity-based probe sequentially targets E1–E2–E3 ubiquitin enzymes. Nat. Chem. Biol. 2016. 16 May Advance Online Publication

    DOI: 10.1038/NCHEMBIO.2084

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