Biotin-Ahx-Ub-Dha

Biotin-Ahx-Ub-Dha

details

UbiQ-102 is a new and first of its kind activity based probe for Ub E1, E2 and (HECT/RBR) E3 ligases [Mulder et al. 2016]. It is based on the Ub sequence in which the C-terminal Gly76 has been replaced by a dehydroalanine (Dha) residue. The N-terminus is labeled with biotin; an aminohexanoic acid (Ahx) linker is used to create extra space between the biotin and Ub protein for efficient access of biotin binding entities. It has been prepared by total chemical synthesis and is therefore well-defined in terms of biotinylation site.

UbiQ-102 is processed in a native manner by Ub E1, E2 and (HECT/RBR) E3 ligases and during this process it forms an electrophilic intermediate that can react with the active site Cys residue of the E1, E2 and (HECT/RBR) E3 enzyme, thereby creating a covalent bond (see figure).

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View FileUbiQ-102 Productsheet B01042016-001    Size: (222.42 KB)
crystallizationpull downpurificationwestern blotphenotypic protein profilingtarget inhibitor profiling

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mg
name Biotin-Ahx-Ub-Dha
product code UbiQ-102
Applications crystallization, pull down, purification, western blot, phenotypic protein profiling, target inhibitor profiling
target E1/E2/E3
source synthetic, human sequence
shipping lyophilized powder
purity ≥95% by RP-HPLC
molecular weight 8.95 kDa
storage Powder at −20°C; solution at −80°C. Protect from light. Please avoid multiple freeze/thaw cycles.
sample preparation For detailed sample preparation see product sheet.
regulatory statement For laboratory research use only, not for use in humans.
  • Triple E poster

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  • Mulder, A., et al.

    Mulder, A., et al. A cascading activity-based probe sequentially targets E1–E2–E3 ubiquitin enzymes. Nat. Chem. Biol. 2016. 16 May Advance Online Publication

    DOI: 10.1038/NCHEMBIO.2084

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