Nedd8-Dha
an activity based probe for Nedd8 E1, E2 and (HECT/RBR) E3 ligases
Additional information
| Weight | 0.005 kg |
|---|---|
| catalogue | |
| Applications | |
| target | |
| source | |
| shipping | |
| purity | |
| molecular weight | |
| storage | Powder at −20°C; solution at −80°C. Protect from light. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
€300.00
- Description
- Additional information
- references
Description
Nedd8-Dha (UbiQ-105) is a first of its kind activity based probe for Nedd8 E1, E2 and E3 ligases [Mulder et al. 2016]. It is based on the Nedd8 sequence in which the C-terminal Gly76 has been replaced by a dehydroalanine (Dha) residue.
UbiQ-105 is processed in a native manner by E1, E2 and E3 ligases and during this process it forms an electrophilic intermediate that can react with the active site Cys residue of the E1, E2 and E3 enzyme, thereby creating a covalent bond (see figure).
Additional information
| Weight | 0.005 kg |
|---|---|
| catalogue | |
| Applications | |
| target | |
| source | |
| shipping | |
| purity | |
| molecular weight | |
| storage | Powder at −20°C; solution at −80°C. Protect from light. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
Mulder et al., A cascading activity-based probe sequentially targets E1–E2–E3 ubiquitin enzymes. Nature Chem Bio (2016) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5108872/