K63 diUb PA

K63 diUb PA


K63 diUb PA (UbiQ-114) is a potent, irreversible and specific inhibitor based on K63 linked diUb that targets the S2 pocket of deubiquitinating enzymes (DUBs). The C-terminus of the proximal Ub is equipped with the propargyl amide (PA) electrophile and the isopeptide bond between the two Ub proteins is replaced by a DUB resistant mimic.

This DUB activity based probe can be used for activity profiling experiments and structural studies. Please note the native distance between the proximal and distal Ub is preserved as much as possible. UbiQ-114 has been prepared by total chemical synthesis.

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View FileUbiQ-114 Productsheet B01082015-001    Size: (240.31 KB)
crystallizationpull downpurificationwestern blotphenotypic protein profiling

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name K63 diUb PA
product code UbiQ-114
Applications crystallization, pull down, purification, western blot, phenotypic protein profiling
target DUB
source human sequence, synthetic
shipping n/a
purity ≥90%
molecular weight 17.1 kDa
storage upon arrival, powder at −20°C, solution at −80°C.
sample preparation For detailed sample preparation see product sheet.
regulatory statement For laboratory research use only, not for use in humans.
    • Galardy et al.

      Galardy, P., et al. Mechanism-based proteomics tools based on ubiquitin and ubiquitin-like proteins: crystallography, activity profiling, and protease identification. Methods in Enzymology. 399, 120-131 (2005).



    • Ekkebus et al.

      Ekkebus, R., et al. On terminal alkynes that can react with active-site cysteine nucleophiles in proteases. J. Am. Chem. Soc. 135, 2867-2870 (2013). 


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