Biotin-K63 diUb-VME
activity-based probe for deubiquitinating enzymes (DUBs) based on K63 linked diUb, labeled with biotin on the N-terminus
Additional information
| Weight | 0.05 kg |
|---|---|
| aliquot size | |
| Applications | Crystallization, Pull down, Purification, Western Blot, Phenotypic protein profiling |
| target | |
| source | |
| purity | |
| molecular weight | |
| storage | |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
€350.00
- Description
- Additional information
- references
Description
UbiQ-115 is an activity-based probe for deubiquitinating enzymes (DUBs) based on K63 linked diUb. Here Lys63 has been replaced by a diaminobutyric acid residue equipped with a vinyl methyl amide warhead – the Dab(VME) type of structure is a DUB reactive mimic of the native isopeptidic linked Lys(Gly) residue. DUB activity based probe can be used for DUB activity profiling experiments and structural studies. The native distance between the proximal and distal Ub is preserved as much as possible in UbiQ-115. The N-terminus of the distal Ub is labeled with biotin; an aminohexanoic acid (Ahx) linker creates extra space for efficient access of biotin binding entities.
Additional information
| Weight | 0.05 kg |
|---|---|
| aliquot size | |
| Applications | Crystallization, Pull down, Purification, Western Blot, Phenotypic protein profiling |
| target | |
| source | |
| purity | |
| molecular weight | |
| storage | |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
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http://www.ncbi.nlm.nih.gov/pubmed/15531586
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http://www.ncbi.nlm.nih.gov/pubmed/23011887
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http://www.ncbi.nlm.nih.gov/pubmed/22118674
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http://www.ncbi.nlm.nih.gov/pubmed/24623714
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http://www.ncbi.nlm.nih.gov/pubmed/24364494
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http://www.ncbi.nlm.nih.gov/pubmed/24225431
Iphöfer, A., et al. Profiling ubiquitin linkage specificities of deubiquitinating enzymes with branched ubiquitin isopeptide probes. Chembiochem 13, 1416-1420 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/22689415
McGouran, J.F., et al. Deubiquitinating enzyme specificity for ubiquitin chain topology profiled by di-ubiquitin activity probes. Chem. Biol. 20, 1447-1455 (2013).
http://www.ncbi.nlm.nih.gov/pubmed/24290882