SUMO1-Dha (UbiQ-116) (Dha= dehydroalanine) is a unique first of its kind activity based probe for the SUMO ligation pathway. It is based on the SUMO1 sequence in which the C-terminal Gly has been replaced by a dehydroalanine residue. The native Cys52 has been mutated to a Ser residue. UbiQ-116 is processed in a native manner by SUMO E1, E2 and E3 ligases and during this process it forms an electrophilic intermediate that can react with the active site Cys residue of the E1, E2 and E3 enzymes, thereby creating a covalent bond (see figure).

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View FileUbiQ-116 Productsheet B01072015-001    Size: (208.68 KB)
crystallizationwestern blot

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name SUMO1-Dha
product code UbiQ-116
Applications crystallization, western blot
target E1/E2/E3
source human sequence, synthetic
shipping lyophilized powder
purity ≥95% by RP-HPLC
molecular weight 11.1 kDa
storage Powder at −20°C; solution at −80°C. Protect from light. Please avoid multiple freeze/thaw cycles.
sample preparation For detailed sample preparation see product sheet.
regulatory statement For laboratory research use only, not for use in humans.
    • Mulder, A., et al.

      Mulder, A., et al. A cascading activity-based probe sequentially targets E1–E2–E3 ubiquitin enzymes. Nat. Chem. Biol. 2016. 16 May Advance Online Publication

      DOI: 10.1038/NCHEMBIO.2084

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