SUMO2-Dha

SUMO2-Dha

details

SUMO2-Dha (UbiQ-117) (Dha= dehydroalanine) is a unique first of its kind activity based probe for the SUMO ligation pathway. It is based on the SUMO2 sequence in which the C-terminal Gly has been replaced by a dehydroalanine residue. The native Cys48 has been mutated to a Ser residue. UbiQ-117 is processed in a native manner by SUMO E1, E2 and E3 ligases and during this process it forms an electrophilic intermediate that can react with the active site Cys residue of the E1, E2 and E3 enzymes, thereby creating a covalent bond (see figure).

Product Attachments

View FileUbiQ-117 Productsheet B01072015-002    Size: (208.47 KB)
crystallizationwestern blot

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mg
name SUMO2-Dha
product code UbiQ-117
Applications crystallization, western blot
target E1/E2/E3
source synthetic, human sequence
shipping lyophilized powder
purity ≥95% by RP-HPLC
molecular weight 10.6 kDa
storage Powder at −20°C; solution at −80°C. Protect from light. Please avoid multiple freeze/thaw cycles.
sample preparation For detailed sample preparation see product sheet.
regulatory statement For laboratory research use only, not for use in humans.
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