Ub-Rh110MP
a quenched, fluorescent substrate for ubiquitin proteases, releasing a brighter Rh110MP dye upon DUB cleavage than UbRh110Gly
Additional information
| Weight | 0.005 kg |
|---|---|
| aliquot size | |
| bulk | |
| Applications | |
| target | |
| source | human sequence, synthetic |
| shipping | |
| purity | |
| molecular weight | |
| storage | |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
€250.00
- Description
- Additional information
- references
Description
Ub-Rh110MP (UbiQ-126) is a quenched, fluorescent substrate for deubiquitylases (DUBs). Cleavage of the amide bond between Gly76 of ubiquitin and the Rhodamine110 moiety releases the highly fluorescent Rh110-morpholinecarbonyl which exhibits a higher fluorescence intensity than the classical Rh110Gly fluorophore of Ub-Rh110Gly (UbiQ-002). UbiQ-126 is prepared by total chemical synthesis.
Additional information
| Weight | 0.005 kg |
|---|---|
| aliquot size | |
| bulk | |
| Applications | |
| target | |
| source | human sequence, synthetic |
| shipping | |
| purity | |
| molecular weight | |
| storage | |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
Hassiepin, U., et al. A sensitive fluorescence intensity assay for deubiquitinating proteases using ubiquitin-rhodamine 110-glycine as substrate. Anal. Biochem. 371, 201-207 (2007).
http://www.ncbi.nlm.nih.gov/pubmed/17869210
El Oualid, F., et al. Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin. Angewandte Chemie Int. Ed. 49, 10149-10153 (2010).
http://www.ncbi.nlm.nih.gov/pubmed/21117055
Lavis, L.D., et al. Fluorogenic label for biomolecular imaging. ACS Chem. Biol. 1, 252-260 (2006).
http://www.ncbi.nlm.nih.gov/pubmed/17163679
Terentyeva, T.G., et al. Morpholinecarbonyl-Rhodamine 110 based substrates for the determination of protease activity with accurate kinetic parameters. Bioconj. Chem. 22, 1932-1938 (2011).
http://www.ncbi.nlm.nih.gov/pubmed/21905728