UbiQ-131 is a new and first of its kind activity based probe for Ub E1, E2 and (HECT/RBR) E3 ligases [Mulder et al. 2016]. It is based on the Ub sequence in which the C-terminal Gly76 has been replaced by a dehydroalanine (Dha) residue. The N-terminus is labeled with the green fluorescent 5-carboxyrhodamine110 dye (cRh110). It has been prepared by total chemical synthesis and is therefore well-defined in terms of dye site.

UbiQ-131 is processed in a native manner by Ub E1, E2 and (HECT/RBR) E3 ligases and during this process it forms an electrophilic intermediate that can react with the active site Cys residue of the E1, E2 and (HECT/RBR) E3 enzyme, thereby creating a covalent bond (see figure).

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View FileUbiQ-131 Productsheet B01112015-001    Size: (222.15 KB)
phenotypic protein profilingcompound library screeningtarget inhibitor profiling

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name 5-carboxyRh110-Ub-Dha
product code UbiQ-131
Applications phenotypic protein profiling, compound library screening, target inhibitor profiling
target E1/E2/E3
source synthetic, human sequence
shipping lyophilized powder
purity ≥95% by RP-HPLC
molecular weight 8.85 kDa
storage Powder at −20°C; solution at −80°C. Protect from light. Please avoid multiple freeze/thaw cycles.
sample preparation For detailed sample preparation see product sheet.
regulatory statement For laboratory research use only, not for use in humans.
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  • Mulder, A., et al.

    Mulder, A., et al. A cascading activity-based probe sequentially targets E1–E2–E3 ubiquitin enzymes. Nat. Chem. Biol. 2016. 16 May Advance Online Publication

    DOI: 10.1038/NCHEMBIO.2084

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