Mca-KKVAPYPME-Dap(Dnp)–NH2 | proteasome activity assay reagent

Mca-KKVAPYPME-Dap(Dnp)–NH2 | proteasome activity assay reagent


UbiQ-141, LF2 peptide assay reagent is a long fluorogenic reagent for monitoring ATP dependant protease activity like in the 26S proteasome and ClpP protease. [Jastrab et al.] Because the LF2 peptide is larger than standard tri/tetra peptide based assay reagents, LF2 is not processed by the 20S CP alone and activating factors are required.


UbiQ-141 | Key Characteristics

  • only processed by activated proteasomes [Jastrab et al.]
  • exhibits a much more pronounced stimulation of proteasome activity than tetrapeptide based proteasome activity assay reagents [Jastrab et al.]

  • processed by human and bacterial proteasomes 


Product Attachments

View FileUbiQ-141 Productsheet B01052016-001

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name Mca-KKVAPYPME-Dap(Dnp)–NH2 | proteasome activity assay reagent
product code UbiQ-141
Applications n/a
target Proteasome
source synthetic
shipping lyophilized powder
purity ≥95% by RP-HPLC
molecular weight found 1.53 kDa
storage powder at −20°C; solution at −80°C. Please avoid multiple freeze/thaw cycles.
sample preparation For detailed sample preparation see product sheet.
regulatory statement For laboratory research use only, not for use in humans.
  • Jastrab, J. B., et al.

    An adenosine triphosphate-independent proteasome activator contributes to the virulence of Mycobacterium tuberculosis. Proc Natl Acad Sci USA. 127, E1763-E1772 (2015),  DOI: 10.1073/pnas.1423319112

  • Smith DM et al.

    Docking of the proteasomal ATPases' carboxyl termini in the 20S proteasome's alpha ring opens the gate for substrate entry. Mol Cell. 27 731-744 (2007). DOI: 10.1016/j.molcel.2007.06.033

  • Smith DM et al.

    ATP binding to PAN or the 26S ATPases causes association with the 20S proteasome, gate opening, and translocation of unfolded proteins. Mol Cell. 20 687-698 (2005). DOI: 10.1016/j.molcel.2005.10.019

  • Bai et al.

    Structural analysis of the dodecameric proteasome activator PafE in Mycobacterium tuberculosis. Proc. Natl. Acad. Sci. USA. 127, E1983-1992 (2015) DOI: 10.1073/pnas.1512094113 

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