Ub photoLeu73

Ub photoLeu73

details

UbiQ-154 is a new crosslinking reagent based on ubiquitin in which Leu73 is replaced by the photoreactive analog photoleucine (photoLeu). Upon irradiation of the diazirine with UV light, a very reactive carbene species is formed. Unspecific binding is minimal for carbene based crosslinking because carbenes are quenched quickly by water, meaning only residues that are very nearby (and thereby contribute to tight binding) will react covalently. Thus any unbound UbiQ-154 will react with water before being able to undergo non-specific reactions with other proteins.

Product Attachments

View FileUbiQ-154 Productsheet B01032017-001
crystallizationpull downwestern blotMS

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mg
name Ub photoLeu73
product code UbiQ-154
Applications crystallization, pull down, western blot, MS
target DUB, E1/E2/E3, Proteasome
source human sequence, synthetic
shipping lyophilized powder
purity >95% by RP-HPLC and SDS-PAGE
molecular weight 8.58 kDa
storage upon arrival powder at −20°C; solution at −80°C.
sample preparation For detailed sample preparation see product sheet.
regulatory statement For laboratory research use only, not for use in humans.