UbiQ-159 (Dha= dehydroalanine) is a new and first of its kind activity based probe for the SUMO ligation pathway. [Mulder et al. 2016] It is based on the SUMO3 sequence in which the C-terminal Gly has been replaced by a dehydroalanine residue. The N-terminus is labeled with biotin; an aminohexanoic acid (Ahx) linker is used to create extra space between the biotin and Ub protein for efficient access of biotin binding entities. It has been prepared by total chemical synthesis and is therefore well-defined in terms of biotinylation site.

UbiQ-159 is processed in a native manner by SUMO E1, E2 and E3 ligases and during this process it forms an electrophilic intermediate that can react with the active site Cys residue of the E1, E2 and E3 enzymes, thereby creating a covalent bond.

Product Attachments

View FileUbiQ-159 Productsheet B01042016-001
crystallizationpull downpurificationwestern blotphenotypic protein profilingtarget inhibitor profiling

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name Biotin-Ahx-SUMO2-Dha
product code UbiQ-159
Applications crystallization, pull down, purification, western blot, phenotypic protein profiling, target inhibitor profiling
target E1/E2/E3
source synthetic, human sequence
shipping lyophilized powder
purity ≥95% by RP-HPLC
molecular weight 10.94 kDa
storage Powder at −20°C; solution at −80°C. Protect from light. Please avoid multiple freeze/thaw cycles.
sample preparation For detailed sample preparation see product sheet.
regulatory statement For laboratory research use only, not for use in humans.
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