HA-Ahx-Ahx-Ub-VS (UbiQ-187) is a potent and specific inhibitor of deubiquitinating enzymes (DUBs) based on a C-terminal electrophilic vinyl sulfone (VS) group. UbiQ-187 can be used for activity profiling experiments and determining DUB inhibitor specificity. UbiQ-187 contains an N-terminal HA-tag (YPYDVPDYA), which is a peptide sequence derived from the influenza hemagglutinin protein and allows for the sensitive identification or purification by anti-HA antibodies and/or anti-HA-agarose. The HA tag is separated from the Ub N-terminus by two aminohexanoic acid (Ahx) linkers for efficient recognition of the tag. To eliminate Met1 oxidation, Met1 is replaced by norleucine, a well validated Met mimic.

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View FileUbiQ-187 Productsheet B01102016-001
crystallizationpurificationwestern blotphenotypic protein profilingDUB activity profiling

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name HA-Ahx-Ahx-Ub-VS
product code UbiQ-187
Applications crystallization, purification, western blot, phenotypic protein profiling, DUB activity profiling
target DUB
source human sequence, synthetic
shipping lyophilized powder
purity ≥95%
molecular weight n/a
storage upon arrival, powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles.
sample preparation For detailed sample preparation see product sheet.
regulatory statement For laboratory research use only, not for use in humans.
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