Biotin-Ahx-Ub-VS (UbiQ-188) is a potent and specific inhibitor of deubiquitinating enzymes (DUBs) based on a C-terminal electrophilic vinyl sulfone (VS) group. UbiQ-188 can be used for activity profiling experiments and determining DUB inhibitor specificity. It is labeled on the N-terminus with a biotin and an aminohexanoic acid (Ahx) linker is introduced to create extra space between the biotin and Ub protein for efficient access of biotin binding entities. To eliminate Met1 oxidation, Met1 is replaced by norleucine, a well validated Met mimic.

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View FileUbiQ-188 Productsheet B01102016-001
crystallizationpurificationwestern blotphenotypic protein profilingDUB activity profiling

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name Biotin-Ahx-Ub-VS
product code UbiQ-188
Applications crystallization, purification, western blot, phenotypic protein profiling, DUB activity profiling
target DUB
source human sequence, synthetic
shipping lyophilized powder
purity ≥95% by RP-HPLC and SDS-PAGE
molecular weight n/a
storage upon arrival, powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles.
sample preparation For detailed sample preparation see product sheet.
regulatory statement For laboratory research use only, not for use in humans.
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