HA-Ahx-Ahx-Ub-VPS (UbiQ-193) is an irreversible inhibitor of deubiquitinating enzymes (DUBs) that is prepared by chemical synthesis. It contains a vinyl pentynyl sulfone (VPS) warhead and N-terminal HA-tag (YPYDVPDYA). The HA tag is separated from the Ub N-terminus by two aminohexanoic acid (Ahx) linkers for efficient recognition of the tag. The VPS electrophile allows for post-labeling modification of covalent DUB::UbiQ-193 complexes by using click chemistry with for example biotin-azide.

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View Fileubiq-193
crystallizationpull downpurificationwestern blotMSNMRphenotypic protein profilingDUB activity profilingcompound library screeningtarget inhibitor profiling

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name HA-Ahx-Ahx-Ub-VPS
product code UbiQ-193
Applications crystallization, pull down, purification, western blot, MS, NMR, phenotypic protein profiling, DUB activity profiling, compound library screening, target inhibitor profiling
target Ubl proteases
source human sequence, synthetic
shipping lyophilized powder
purity ≥95% by HPLC
molecular weight 9.96 kDa
storage upon arrival, powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles.
sample preparation For detailed sample preparation see product sheet.
regulatory statement For laboratory research use only, not for use in humans.