His6-Ahx-Ahx-SUMO2-VPS

His6-Ahx-Ahx-SUMO2-VPS

details

His6-Ahx-Ahx-SUMO2-VPS (UbiQ-237) is an activity-based probe for SUMO proteases that is prepared by total chemical synthesis. It contains a C-terminal vinyl pentynyl sulfone (VPS) electrophile (Figure 1) and an N-terminal His6 sequence which allows for sensitive identification or purification by anti-His6 antibodies and/or anti-His6-agarose. The VPS electrophile allows for post-labeling modification of cross-linked UbiQ-237::SENP complexes by using click chemistry with for example biotin-azide.

Product Attachments

View Fileubiq-237
crystallizationpull downpurificationwestern blotMSNMRphenotypic protein profilingDUB activity profilingcompound library screeningtarget inhibitor profiling

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mg
name His6-Ahx-Ahx-SUMO2-VPS
product code UbiQ-237
Applications crystallization, pull down, purification, western blot, MS, NMR, phenotypic protein profiling, DUB activity profiling, compound library screening, target inhibitor profiling
target Ubl proteases
source human sequence, synthetic
shipping lyophilized powder
purity ≥95% by RP-HPLC and SDS-PAGE
molecular weight n/a
storage upon arrival, powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles.
sample preparation For detailed sample preparation see product sheet.
regulatory statement For laboratory research use only, not for use in humans.