UbiQ-L01 is an explorer panel of all eight native linked di-ubiquitin (diUb) conjugates: M1 (i.e. linear), K6, K11, K27, K29, K33, K48 and K63 linked. The seven isopeptide linked diUb conjugates have been prepared by chemical ligation, the linear (M1) diUb conjugate by recombinant expression.
UbiQ-L01 can be used to:
- investigate the linkage specificity of proteases that cleave the (iso)peptide linkage between two ubiquitin proteins.
- investigate mechanism of binding and recognition of proteins that contain ubiquitin-associated domains / or ubiquitin-interacting motifs (UIMs).