di-ubiquitin chain explorer panel

di-ubiquitin chain explorer panel

details

UbiQ-L01 is an explorer panel of all eight native linked di-ubiquitin (diUb) conjugates: M1 (i.e. linear), K6, K11, K27, K29, K33, K48 and K63 linked. The seven isopeptide linked diUb conjugates have been prepared by chemical ligation, the linear (M1) diUb conjugate by recombinant expression.

UbiQ-L01 can be used to:

  1. investigate the linkage specificity of proteases that cleave the (iso)peptide linkage between two ubiquitin proteins.
  2. investigate mechanism of binding and recognition of proteins that contain ubiquitin-associated domains / or ubiquitin-interacting motifs (UIMs).

Product Attachments

View FileUbiQ-L01 Productsheet B01052014-001
crystallizationphenotypic protein profiling

Qty Available: 98

€300.00

Qty Available: 98

€300.00

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mg
name di-ubiquitin chain explorer panel
product code UbiQ-L01
Applications crystallization, phenotypic protein profiling
target DUB
source human, synthetic
shipping lyophilized powder
purity ≥95% by RP-HPLC and LC-MS analysis, ≥95% by SDS-PAGE
molecular weight 17112 Da
storage Powder at −20°C; solution at −80°C. Please avoid multiple freeze/thaw cycles.
sample preparation For detailed sample preparation see product sheet.
regulatory statement For laboratory research use only, not for use in humans.
  • Faesen et al.

    Faesen, A.C., et al. The Differential Modulation of USP Activity by Internal Regulatory Domains, Interactors and Eight Ubiquitin Chain Types. Chem. Biol. 18, 1550-1561 (2011). 

    http://www.ncbi.nlm.nih.gov/pubmed/22195557 

  • I. Dikic et al.

    Dikic, I., et al. Ubiquitin-binding domains - from structures to functions. Nat. Rev. Mol. Cell. Biol. 10, 659-671 (2010). 

    http://www.ncbi.nlm.nih.gov/pubmed/19773779

  • J. D. F. Licchesi et al.

    Licchesi, J.D., et al. An ankyrin-repeat ubiquitin-binding domain determines TRABID's specificity for atypical ubiquitin chains. Nat. Struct. Mol. Biol. 19, 62-71 (2012). 

    http://www.ncbi.nlm.nih.gov/pubmed/22157957

  • UbiQ_Chains factsheet download this pdf
  • UbiQ-Probes crystallization overview download this pdf
  • UbiQ's drug discovery expertise overview

     

    download this pdf