di-ubiquitin activity based probe explorer panel

di-ubiquitin activity based probe explorer panel

details

UbiQ-L04 contains seven deubiquitylating enzymes (DUB) activity-based probes based on K6, 11, 27, 29, 33, 48, and 63 linked diUb. These irreversible and specific inhibitors of DUBs can be used for activity-based protein profiling experiments and structural studies. Please note that the native distance between the proximal and distal Ubiquitin is preserved as much as possible in these new diUb probes, giving rise to less artefact binding compared to the native diUb cleavage profile of DUBs. For details see product sheets.

Product Attachments

View FileUbiQ-L04 Productsheet B01022015-001
crystallizationpurification

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€450.00

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€450.00

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mg
name di-ubiquitin activity based probe explorer panel
product code UbiQ-L04
Applications crystallization, purification
target DUB
source human, synthetic
shipping lyophilized powder
purity ±90% by RP-HPLC and SDS-PAGE
molecular weight 17110 Da
storage Powder at −20°C; solution at −80°C. Please avoid multiple freeze/thaw cycles.
sample preparation For detailed sample preparation see product sheet.sired.
regulatory statement For laboratory research use only, not for use in humans.
  • Faesen et al.

    Faesen, A.C., et al. The Differential Modulation of USP Activity by Internal Regulatory Domains, Interactors and Eight Ubiquitin Chain Types. Chem. Biol. 18, 1550-1561 (2011). 

    http://www.ncbi.nlm.nih.gov/pubmed/22195557 

  • Mulder & El Oualid et al.

    Mulder, M.P., et al. A native chemical ligation handle that enables the synthesis of advanced activity-based probes: diubiquitin as a case study. ChemBiochem 15, 946-949 (2014).

    http://www.ncbi.nlm.nih.gov/pubmed/24623714

  • Haj-Yahya et al.

    Haj-Yahya, N., et al. Dehydroalanine-based diubiquitin activity probes. Org. Lett. 16, 540-543 (2014). 

    http://www.ncbi.nlm.nih.gov/pubmed/24364494

  • Li et al.

    Li, G., et al. Activity-based diubiquitin probes for elucidating the linkage specificity of deubiquitinating enzymes. Chem. Commun. 20, 216-218 (2014).

    http://www.ncbi.nlm.nih.gov/pubmed/24225431

  • Iphöfer et al.

    Iphöfer, A., et al. Profiling ubiquitin linkage specificities of deubiquitinating enzymes with branched ubiquitin isopeptide probes. Chembiochem 13, 1416-1420 (2012). 

    http://www.ncbi.nlm.nih.gov/pubmed/22689415

  • McGouran et al.

    McGouran, J.F., et al. Deubiquitinating enzyme specificity for ubiquitin chain topology profiled by di-ubiquitin activity probes. Chem. Biol. 20, 1447-1455 (2013). 

    http://www.ncbi.nlm.nih.gov/pubmed/24290882

  • UbiQ-Probes crystallization overview download this pdf
  • UbiQ_DUB Probes factsheet download this pdf
  • UbiQ's drug discovery expertise overview

     

    download this pdf