UbiQ-018

Me4BodipyFL-Ahx3Leu3VS | fluorescent proteasome probe

details

UbiQ-018, Me4BodipyFL-Ahx3Leu3VS is a fluorescent proteasome activity probe that allows for accurate profiling of proteasomal activity in cell lysates, intact cells, and murine and human patient-derived material, with high sensitivity using SDS-PAGE. The probe allows for direct scanning of the gel for fluorescent emission of the distinct proteasomal subunits and circumvents the use of western-blot analysis.

Due to its suitable biochemical and biophysical properties the fluorescent probe can also be used for confocal laser scanning microscopy and flow cytometry-based experiments.

Product Attachments

UbiQ-018 Productsheet B01092013

crystallizationpull downpurificationwestern blotphenotypic protein profiling

Qty Available: 43

€280.00

aliquot size

€280.00

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name	Me4BodipyFL-Ahx3Leu3VS \| fluorescent proteasome probe
product code	UbiQ-018
Applications	crystallization, pull down, purification, western blot, phenotypic protein profiling
target	Proteasome
source	synthetic
shipping	lyophilized powder
purity	≥95% by RP-HPLC
molecular weight	found 1059 Da, calc 1059 Da
storage	Powder at +4°C; solution at −80°C. Please avoid multiple freeze/thaw cycles.
sample preparation	For detailed sample preparation see product sheet.
regulatory statement	For laboratory research use only, not for use in humans.

activity-based DUB inhibitor profiling using SDS-PAGE download this pdf

Berkers et al.
Berkers, C.R., et al. Profiling proteasome activity in tissue with fluorescent probes. Mol. Pharm. 739, 739-748 (2007).

http://www.ncbi.nlm.nih.gov/pubmed/17708652
Berkers et al.
Berkers, C.R., et al. Probing the specificity and activity profiles of the proteasome inhibitors bortezomib and delanzomib. Mol. Pharm. 9, 1126-1136 (2012).

http://www.ncbi.nlm.nih.gov/pubmed/22432738

UbiQ_DUB Probes factsheet download this pdf
UbiQ's drug discovery expertise overview

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