UbiQ-073 is a fluorescence polarization assay reagent for deISGylating enzymes which is based on a 5-carboxytetramethylrhodamine (TAMRA, exc 550 nm, emi 590 nm) modified Lys-Gly sequence that is linked to (mouse) ISG15 (Interferon stimulated gene 15) via a native isopeptide bond with the lysine side-chain. ISG15 is a ubiquitin-like protein whose expression is increased following stimulation with type 1 Interferons.
A typical substrate concentration is 100 nM. Effective concentrations of deISGylating enzymes depends on specific assay conditions and method of detection.
UbiQ-073 is effectively processed by the known deISGylating enzyme USP18. (see product sheet for more info)