UbiQ-262 is an activity-based probe (ABP) for ISG15 proteases. It is prepared by total chemical synthesis and based on the proximal domain of mouse ISG15. It contains a C-terminal vinyl pentynyl sulfone (VPS) electrophile (Figure 1), allowing for post-labeling modification of cross-linked [UbiQ-262]::[ISG15 protease] complexes by using click chemistry with for example biotin-azide.

Product Attachments

View FileUbiQ-262 Productsheet B01042020-001
crystallizationpull downpurificationwestern blotMSNMRphenotypic protein profilingDUB activity profilingcompound library screeningtarget inhibitor profiling

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name Ac-ISG15prox-VPS
product code UbiQ-262
Applications crystallization, pull down, purification, western blot, MS, NMR, phenotypic protein profiling, DUB activity profiling, compound library screening, target inhibitor profiling
target ISG15
source mouse sequence, proximal domain, synthetic
shipping lyophilized powder
purity ≥95%
molecular weight n/a
storage upon arrival, powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles.
sample preparation For detailed sample preparation see product sheet.
regulatory statement For laboratory research use only, not for use in humans.